| Alley | Hip | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|---|
| 1A | 7 | Barbour, Samantha | Warner | 3:37.84 | |
| 2A | 5 | Ream, Koree | Webber | 2:38.21 | |
| 3A | 3 | Edens, Sarah | Unattached | 2:29.00 | |
| 4A | 1 | Levine, Marina | SR | Unattached | 2:20.12 |
| 4B | 9 | Whitfield, Kathi | SR | Webber | NT |
| 5A | 2 | Meneses, Alex | Embry-Riddle | 2:27.29 | |
| 6A | 4 | Rybinski, Gabriella | 8 | Unattached | 2:38.00 |
| 7A | 6 | Solomon, Larica | Webber | 2:52.88 | |
| 8A | 8 | Fernandez, Lua | Webber | NT |