| Lane | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|
| 1 | Czurak, Mackenzie | SO | Winter Springs HS | 11:22.90 |
| 2 | Coddington, Jennifer | JR | Winter Park HS | 11:37.84 |
| 3 | Adams, Grace | SO | Winter Park HS | 11:55.20 |
| 4 | Dietz, Stephanie | SO | Winter Park HS | 12:10.00 |
| 5 | McDonnell, Claire | SO | Father Lopez Catholic HS | 12:10.17 |
| 6 | Demetriades, Olivia | JR | Trinity Prep | 12:30.58 |
| 7 | Muse, Kamryn | JR | Hagerty HS | 12:50.00 |
| 8 | Messenger, Kayla | JR | Hagerty HS | 13:24.59 |
| 9 | Zagorini, Fioanna | SO | Mount Dora HS | 14:10.21 |
| 10 | Mawad, Gabby | SR | The Master's Academy (Oviedo) | 16:10.50 |
| 11 | Albright, Olivia | SR | Trinity Prep | NT |
| 12 | Doyle, Susie | 9 | Winter Springs HS | NT |
| 13 | Karamaniska, Katerina | 9 | Sanford Seminole HS | NT |
| 14 | Skidmore, Isabella | 9 | Sanford Seminole HS | NT |
| 15 | Wilhelm, Emily | 9 | Trinity Prep | NT |