| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | JACKSON, Shyann | Poinciana HS | 1:18.00 |
| 2 | BARNETT, Aliyah | Poinciana HS | 1:21.00 |
| 3 | ISAAC, Akemah | Poinciana HS | 1:21.00 |
| 4 | MAYES, Shyanne | West Orange HS | 1:39.77 |
| 5 | WHITE, Melanie | Winter Park HS | 1:40.53 |
| 6 | MICHEL, Bensdjadini | Oak Ridge HS | 1:41.00 |
| 7 | THOMAS, Shaniya | Cocoa HS | 1:42.46 |
| 8 | GIBBONS, Rafaella | Winter Park HS | 1:43.57 |
| 9 | JOHNSON, Gabrielle | Winter Park HS | 1:43.60 |
| 10 | ALVARDO, Aliana | Colonial HS | 1:45.00 |
| 11 | VESTOSKY, Ginna | Colonial HS | 1:45.00 |
| 12 | RICHARDSON, Calyn | Colonial HS | 1:46.00 |
| 13 | AUGUSTE, Yvonika | West Orange HS | 1:47.82 |
| 14 | BAKER, Azaria | Edgewater HS | 1:50.00 |
| 15 | DOUGLAS, Shayla | West Orange HS | 1:50.00 |
| 16 | ST. ANGELO, Rachel | Lake Howell HS | 1:50.21 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | METAYER, Ghaina | Edgewater HS | 1:51.00 |
| 2 | SAINTINE, Mysaul | Oak Ridge HS | 1:53.70 |
| 3 | HARRIS, Alexa | Edgewater HS | 1:54.00 |
| 4 | SPEISER, Willow | Celebration HS | 1:55.00 |
| 5 | WILLIAMS, Saphire | Cocoa HS | 1:55.00 |
| 6 | PODBORSKY, Melanie | Celebration HS | 1:58.00 |
| 7 | TEOW, Jaden | Celebration HS | 1:59.00 |
| 8 | TENNEY, Dysis | Cocoa HS | 2:00.00 |
| 9 | OJEDA, Dejah | University HS | 2:00.51 |
| 10 | GORDON, Jhanel | Oak Ridge HS | NT |
| 11 | HERNANDEZ, Tatiana | Lake Howell HS | NT |
| 12 | LACROIX, Ayanna | First Academy (Orlando) | NT |
| 13 | SULLIVAN, Eva | First Academy (Orlando) | NT |
| 14 | VAHEY, Mackenzie | Orange City University HS | NT |
| 15 | WILLIAMS, Brooke | Orange City University HS | NT |