| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | CHARLES, Jaariq | Cocoa HS | 1:22.00 |
| 2 | RUTH, Sy'Rkia | Cocoa HS | 1:25.00 |
| 3 | MODESTE, Joshua | Oak Ridge HS | 1:26.65 |
| 4 | PREDDIE, Rajay | West Orange HS | 1:27.68 |
| 5 | LEWIS, John | West Orange HS | NT |
| 6 | DIX, Avion | Colonial HS | 1:30.00 |
| 7 | MOREAU, Darien | Edgewater HS | 1:30.00 |
| 8 | STRATTON, Will | Edgewater HS | 1:30.00 |
| 9 | MONTALVO, Moises | Colonial HS | 1:30.10 |
| 10 | YOUNG, Branden | West Orange HS | 1:30.28 |
| 11 | CLARKE, Jehu | Edgewater HS | 1:35.00 |
| 12 | MCFADDEN, Devyn | Colonial HS | 1:35.00 |
| 13 | GENTRY, Devon | Orange City University HS | 1:35.22 |
| 14 | STRIKE, Cote | Celebration HS | 1:37.28 |
| 15 | VAN DE MEENE, Mark | Winter Park HS | 1:40.00 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | URIOSTEGUI, Juan | Celebration HS | 1:48.00 |
| 2 | GODOY, Raycel | Celebration HS | 1:50.00 |
| 3 | RAMOS, Jose | Poinciana HS | 1:50.00 |
| 4 | TATIS, Joshua | Poinciana HS | 1:58.00 |
| 5 | WHISONANT, Denzel | Poinciana HS | 2:00.00 |
| 6 | FREED, Sam | Winter Park HS | 1:41:00.00 |
| 7 | MANZOOR, Ashhad | Winter Park HS | 1:43:00.00 |
| 8 | ALEXANDER, Christian | Lake Howell HS | NT |
| 9 | CLARK, Erin | Oak Ridge HS | NT |
| 10 | DEJESUS, Danniel | University HS | NT |
| 11 | GREEN, Tyler | First Academy (Orlando) | NT |
| 12 | MIDDLETON, Jalen | First Academy (Orlando) | NT |
| 13 | PARRY, Benjamin | Orange City University HS | NT |
| 14 | PHILIPPE, Belodeson | Oak Ridge HS | NT |
| 15 | SONNIE, Thomas | Lake Howell HS | NT |
| 16 | TIPTON, Jacob | Orange City University HS | NT |