| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | QUINTANA, Noah | Lake Minneola HS | 4:26.55 | Verified |
| 2 | BELL, Bryce | Lyman HS | 4:26.89 | Verified |
| 3 | HIRSCH, Walker | Satellite High School | 4:29.10 | Verified |
| 4 | VALIQUETTE, Ryder | Montverde Academy | 4:37.59 | Verified |
| 5 | BASFORD, Nathaniel | Satellite High School | 4:39.00 | Unverified: Deltona Invite |
| 6 | KOSHY, Rahul | Satellite High School | 4:43.00 | Unverified: Deltona Invite |
| 7 | O'BRIEN, Gavin | Astronaut HS | 4:43.12 | Verified |
| 8 | O'MALLEY, Garrett | Hagerty HS | 4:43.63 | Verified |
| 9 | EBERT, Nicholas | Hagerty HS | 4:46.11 | Verified |
| 10 | LAWSON, Josh | Venice HS | 4:50.07 | Verified |
| 11 | LABOY, Gabriel | Tavares HS | 4:50.34 | Verified |
| 12 | SWEIDERK, Ben | Venice HS | 4:52.15 | Verified |
| 13 | PAUTIENUS, David | Lake Minneola HS | 4:52.27 | Verified |
| 14 | KRESS, Gabriel | Lake Nona HS | 4:52.90 | Verified |
| 15 | ABBOTT, Ty | Venice HS | 4:55.34 | Unverified: |
| 16 | ARNOLD, Aiden | Lake Nona HS | 4:56.42 | Verified |
| 17 | CHANDLER, Clayton | Tavares HS | 4:56.53 | Verified |
| 18 | WITTMAYER, Jack | Lake Nona HS | 4:57.21 | Verified |
| 19 | PETRO, Campbell | Lake Highland Prep HS | 4:57.87 | Verified |
| 20 | WILLIAMSON, Jack | Lyman HS | 4:58.00 | Unverified: |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | PHILLIPS, Grayson | Lake Highland Prep HS | 4:58.21 | Verified |
| 2 | TORRES, Adrian | Lyman HS | 4:58.99 | Verified |
| 3 | KENOYER, Ty | Lake Highland Prep HS | 5:04.61 | Verified |
| 4 | NESMITH, Nico | Montverde Academy | 5:17.10 | Verified |
| 5 | BARRETTO, Adrian | Hagerty HS | 5:19.75 | Verified |
| 6 | REED, Samuel | South Sumter HS | 5:30.00 | Unverified: |
| 7 | COOK, William | Montverde Academy | 5:32.47 | Verified |
| 8 | BENJAMIN, Jalen | North Marion HS | 5:36.33 | Verified |
| 9 | DEROSA, Jaiden | North Marion HS | 5:39.29 | Verified |
| 10 | DUBON, Josue | North Marion HS | 5:40.44 | Verified |
| 11 | WHITE, Martin | The Geneva School | 5:48.31 | Verified |
| 12 | DEARMAN, Levi | South Sumter HS | 5:50.00 | Unverified: |
| 13 | BISINIERE, Jackson | Bishop Moore HS | NT | Unverified: |
| 14 | DEVOSS, Simon | Astronaut HS | NT | Unverified: |
| 15 | DELGADO-MATIAS, Jose | Bishop Moore HS | NT | Unverified: |
| 16 | GOTHAM, Jeffrey | Lake Minneola HS | NT | Unverified: |
| 17 | HEDRICK, Luke | Bishop Moore HS | NT | Unverified: |
| 18 | HOAG, Riley | Tavares HS | NT | Unverified: |
| 19 | MCHUGH, Brandon | Astronaut HS | NT | Unverified: |