| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | CROBONS, Catalina | Braden River HS | 1:06.00 | Unverified: |
| 2 | CHAMBERS, Abigail | Port Charlotte HS | 1:04.55 | Verified |
| 3 | PARSONS, Jenny | Lemon Bay HS | 1:03.00 | Unverified: |
| 4 | MCDOWELL, Carmen | Booker HS | 1:00.00 | Unverified: |
| 5 | CASIMIR, Charneycar | Bayshore HS | 1:00.00 | Unverified: |
| 6 | MENDEZ, Sarah | North Port HS | 1:04.10 | Unverified: |
| 7 | PROCOPIO, Capri | Booker HS | 1:05.00 | Unverified: |
| 8 | COTE, Sarah | Lemon Bay HS | 1:06.00 | Unverified: |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | HARRIS, KIara | Booker HS | 1:10.00 | Unverified: |
| 2 | LOCKER, Kristen | Port Charlotte HS | 1:10.00 | Unverified: |
| 3 | BORER, Charleze | North Port HS | 1:09.87 | Unverified: |
| 4 | BURWOOD, Abbey | Out of Door Academy | 1:06.27 | Verified |
| 5 | RAGAZZONE, Lauren | Lemon Bay HS | 1:07.00 | Unverified: |
| 6 | INGMAN, Shannon | Port Charlotte HS | 1:10.00 | Unverified: |
| 7 | BUSI, Isabella | Braden River HS | 1:10.00 | Unverified: |
| 8 | LEKIC, Alexis | North Port HS | 1:13.70 | Unverified: |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | WALL, McKenna | Island Coast HS | NT | Unverified: |
| 2 | PAILLE, Taylor | Community Christian School (Port Charlotte) | NT | Unverified: |
| 3 | PRIOVOLOS, Olivia | Sarasota Riverview HS | NT | Unverified: |
| 4 | WIDEMAN, Amari | Sarasota Riverview HS | NT | Unverified: |
| 5 | SWIFT, Meredith | Sarasota Riverview HS | NT | Unverified: |
| 6 | LACOURSE, Tatyana | Bishop Verot HS | NT | Unverified: |
| 7 | AUSSET, Elena | Bishop Verot HS | NT | Unverified: |
| 8 | OGDEN, Natalie | Bishop Verot HS | NT | Unverified: |