| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | THOMPSON, Kobe | JR | Satellite High School | 4:48.24 | Verified |
| 2 | COLLINS, Sam | SR | Winter Springs HS | 4:50.86 | Verified |
| 3 | EDWARDS, Nathan | JR | First Academy (Orlando) | 4:55.10 | Verified |
| 4 | ALLEN, Mitchell | SR | First Academy (Orlando) | 4:59.99 | Verified |
| 5 | STEGER, Colby | SO | Satellite High School | 5:04.54 | Verified |
| 6 | CUMMINS, Walker | SO | Satellite High School | 5:05.00 | Unverified: |
| 7 | GOODSELL, Hayden | 9 | Lake Howell HS | 5:05.00 | Unverified: Wildcat Open |
| 8 | O'TOOLE, Troy | SO | Olympia HS | 5:06.84 | Verified |
| 9 | GERBER, Michael | SR | Olympia HS | 5:09.59 | Verified |
| 10 | MINK, Noah | SR | Lake Howell HS | 5:10.35 | Verified |
| 11 | ROWE, Carter | SO | First Academy (Orlando) | 5:12.30 | Unverified: Bob Hosher |
| 12 | GARVER, Jay | 9 | Foundation Academy | 5:17.90 | Verified |
| 13 | SANTIAGO, Celestino | SO | Orlando Christian Prep | 5:28.66 | Verified |
| 14 | SONNIE, Thomas | SR | Lake Howell HS | 5:30.85 | Verified |
| 15 | SMITH, Ben | JR | Foundation Academy | 5:40.57 | Verified |
| 16 | LEE, John-Carlo | 9 | Dr. Phillips HS | 5:54.43 | Verified |
| 17 | CLARK, Erin | SO | Oak Ridge HS | NT | Unverified: |
| 18 | DORT, Retz | 9 | Oak Ridge HS | NT | Unverified: |
| 19 | JEAN, Jazzmy | SR | Evans HS | NT | Unverified: |
| 20 | KOMATSU, Kai | 9 | Olympia HS | NT | Unverified: |
| 21 | LAROSILIERE, Orldiny | SO | Orlando Christian Prep | NT | Unverified: |
| 22 | MARCELIN, Kenley | 9 | Oak Ridge HS | NT | Unverified: |
| 23 | SEMLALI, Ilyas | 9 | Dr. Phillips HS | NT | Unverified: |
| 24 | STONE, Brennan | SR | Trinity Christian Academy (Deltona) | NT | Unverified: |