| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CZURAK, Mackenzie | SO | Winter Springs HS | 5:16.89 | Verified |
| 2 | CRIST, Caroline | SR | First Academy (Orlando) | 5:23.06 | Verified |
| 3 | KETELSEN, Hannah | SO | Lake Howell HS | 5:43.01 | Verified |
| 4 | CRIST, Isabel | SO | First Academy (Orlando) | 5:50.40 | Verified |
| 5 | FLAMENT, Alice | SO | Olympia HS | 6:02.92 | Verified |
| 6 | WEBER, Morgan | SO | Olympia HS | 6:19.01 | Verified |
| 7 | ZAPATA, Isabella | 9 | Lake Howell HS | 6:20.00 | Unverified: |
| 8 | MORROW, Rebekah | JR | Trinity Christian Academy (Deltona) | 6:23.27 | Verified |
| 9 | JEAN-BAPTISTE, Briana | JR | Oak Ridge HS | 6:33.29 | Verified |
| 10 | LUGO, Gabriela | SO | Olympia HS | 6:33.64 | Verified |
| 11 | ANDERSEN, Stacie | SR | Lake Howell HS | 7:01.17 | Verified |
| 12 | GEORGES, Rebecca | SO | Oak Ridge HS | NT | Unverified: |
| 13 | HOPKE, Hannah | JR | First Academy of Leesburg | NT | Unverified: |
| 14 | PHAM, Stepheny | SR | Dr. Phillips HS | NT | Unverified: |
| 15 | WIMBUSH, Meilah | SO | Dr. Phillips HS | NT | Unverified: |