| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | VILLANUEVA-GARCIA, Cristina | Bishop Moore HS | 26.77 |
| 2 | HARVEY, Kilani | Jones HS | 26.27 |
| 3 | TOWNSEND, Janiyah | Jones HS | 25.71 |
| 4 | BROWN, Ranecia | Oak Ridge HS | 24.50 |
| 5 | GORDON, Jhanel | Oak Ridge HS | 25.00 |
| 6 | FORTILIEN, Ebone | Dr. Phillips HS | 25.99 |
| 7 | WHITE, Azaria | Jones HS | 26.33 |
| 8 | MICHEL, Robilina | Dr. Phillips HS | 26.80 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | ALEXANDER, Aasha | Dr. Phillips HS | 27.95 |
| 2 | ALI, Amira | Hagerty HS | 27.33 |
| 3 | SMITH, Khadayjha | Oviedo HS | 27.01 |
| 4 | DAWSON, Rebecca | Lake Nona HS | 26.90 |
| 5 | HIGGS, Ashley | Oak Ridge HS | 26.92 |
| 6 | JAMISON, Aja | Hagerty HS | 27.08 |
| 7 | MERENDA, Olivia | Bishop Moore HS | 27.53 |
| 8 | DONOVAN, Melissa | Hagerty HS | 28.76 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | MITCHELL, Imani | Winter Springs HS | 29.51 |
| 2 | KOSMITIS, Kellie | Oviedo HS | 29.22 |
| 3 | COTTER, Jacey | Lake Highland Prep HS | 29.14 |
| 4 | SMITH, Kanya | Oviedo HS | 28.96 |
| 5 | KUZNOF, Kenya | Father Lopez Catholic HS | 29.01 |
| 6 | HUET, Rylee | Winter Springs HS | 29.20 |
| 7 | THERIAULT, Kather | Father Lopez Catholic HS | 29.50 |
| 8 | BAILTON, Flynn | Father Lopez Catholic HS | 30.43 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | EVANS, Maison | Umatilla HS | 33.13 |
| 2 | RUIZ, Kendall | Lake Nona HS | 33.05 |
| 3 | CANAL, Madeline | Trinity Prep | 32.00 |
| 4 | SMITH, Julia | Umatilla HS | 30.47 |
| 5 | WESLEY, Artayia | Bishop Moore HS | 31.49 |
| 6 | GEORGE, Ashley | Lake Highland Prep HS | 32.86 |
| 7 | DESAI, Sonal | Lake Highland Prep HS | 33.10 |
| 8 | PARKER, Grace | Trinity Prep | 33.55 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 3 | ALMONTE, Alexa | Lake Nona HS | NT |
| 4 | MADDEN, Isabella | Umatilla HS | 34.10 |
| 5 | WARREN, Samantha | Trinity Prep | NT |