| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | CSUBAK, Lauren | Saint Leo | 2:18.24 | Embry-Riddle First Chance Last Chance |
| 2 | CHRISTENSEN, Emily | Lynn | 2:20.00 | |
| 3 | IRWIN, Shannon | Tampa | 2:20.00 | |
| 4 | LOCKRIDGE, Julia | Tampa | 2:20.00 | |
| 5 | BIBLE, Alexis | Tampa | 2:21.00 | |
| 6 | BRILL, Monika | UNAT-Tampa | 2:21.00 | |
| 7 | KVITKAUSKAUS, Nicole | Embry-Riddle | 2:21.55 | Embry-Riddle First Chance Last Chance |
| 8 | ROUSSELL, Haley | Saint Leo | 2:23.23 | USF Bulls Invitational |
| 9 | WATKINS, Kassidy | Florida Southern | 2:24.64 | Florida Division 2 Challenge |
| 10 | ROMERO, Celeste | Nova Southeastern | 2:25.00 | |
| 11 | STONEBACK, Sydney | Nova Southeastern | 2:25.00 | |
| 12 | PETRUS, Emily | South Florida | 2:26.00 | |
| 13 | BELLO, Nicole | South Florida | 2:28.00 | |
| 14 | WHIFFEN, Katie | Stetson | 2:29.72 | |
| 15 | MARCIN, Lesly | UNAT-St. Thomas (Fla.) | 2:29.93 | Embry-Riddle First Chance Last Chance |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | CURRAN, Sara | Tampa | 2:30.00 | |
| 2 | HEDDERMAN, Michaela | South Florida | 2:30.00 | |
| 3 | MOSELY, Kianna | South Florida | 2:30.00 | |
| 4 | EARLY, Shannon | Tampa | 2:32.00 | |
| 5 | KACHULIS, Sophia | Tampa | 2:32.00 | |
| 6 | BLACKWELL, Erin | Nova Southeastern | 2:32.61 | Florida Division 2 Challenge |
| 7 | FISHER, Kayla | Palm Beach Atlantic | 2:33.57 | Embry-Riddle First Chance Last Chance |
| 8 | WATTS, Darby | Lynn | 2:35.00 | |
| 9 | WILLS, Mackenzie | Florida Southern | 2:35.00 | |
| 10 | BECK, Natalie | Lynn | 2:35.34 | USF Invitational |
| 11 | MILLER, Jacinda | Valdosta State | 2:35.87 | Emory Classic |
| 12 | SVENSSON, Elin | Florida Tech | 2:36.20 | Embry-Riddle First Chance Last Chance |
| 13 | ROSENER, Olivia | Valdosta State | 2:38.00 | |
| 14 | NAYA, Sofia | UNAT-St. Thomas (Fla.) | 2:38.61 | Embry-Riddle First Chance Last Chance |
| 15 | PERELLA, Lillian | West Virginia Wesleyan | 2:40.00 | |
| 16 | WIETHE, Natalie | West Virginia Wesleyan | 2:40.00 | |
| 17 | YOCUM, Julia | West Virginia Wesleyan | 2:40.00 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | CHAVIS, Jessica | Palm Beach Atlantic | 2:42.54 | Embry-Riddle First Chance Last Chance |
| 2 | MORANTE, Mariana | UNAT-St. Thomas (Fla.) | 2:42.89 | Embry-Riddle First Chance Last Chance |
| 3 | TOMBERLIN, Sydney | Valdosta State | 2:44.21 | North Florida Invitational |
| 4 | FERNANDEZ, Maria | Saint Leo | 2:45.00 | |
| 5 | MCELROY, Courtney | Florida Southern | 2:45.00 | |
| 6 | ORLANDO, Casey | Florida Tech | 2:45.00 | |
| 7 | BOYER, Bryson | Florida Tech | 2:52.59 | Embry-Riddle First Chance Last Chance |
| 8 | MEMBRENO, Escarleth | St. Thomas (Fla.) | 2:56.58 | Embry-Riddle First Chance Last Chance |
| 9 | WILLIAMS, Jocelyn | Unattached | 3:00.00 | |
| 10 | RIOS ROSALES, AnaPaula | Saint Leo | 3:15.89 | |
| 11 | BUDD, Samantha | ETA Coach | NT | |
| 12 | DMYTRUK, Alexandra | Florida Tech | NT | |
| 13 | GRANLUND, Isabelle | Palm Beach Atlantic | NT | |
| 14 | HENNESSEY, Amanda | Lynn | NT | |
| 15 | LYKINS, Jessica | Palm Beach Atlantic | NT | |
| 16 | PILOTO, Daniella | UNAT-St. Thomas (Fla.) | NT | |
| 17 | RICHARDSON, Makayla | Palm Beach Atlantic | NT |