| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | SPANGLER, Emilee | West Virginia Wesleyan | 1:05.00 | |
| 2 | GLASS, Emily | Lynn | 1:03.00 | |
| 3 | LECKENBY, Lauren | Lynn | 1:00.00 | |
| 4 | HYMAN, OSHIN | Warner | 58.51 | |
| 5 | YODER, Chase | Lynn | 1:00.00 | |
| 6 | BLAKE, Laura | West Virginia Wesleyan | 1:01.06 | Brandi Bowen Invitational |
| 7 | CORDIER, China | Tampa | 1:04.00 | |
| 8 | SPENCER, Arianna | Lynn | 1:05.00 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | MORIN, Courtney | Lynn | 1:07.00 | |
| 2 | WHALEN, Lynn | Lynn | 1:07.00 | |
| 3 | BURNS, Kamran | Tampa | 1:06.00 | |
| 4 | WALKER, Machell | St. Thomas (Fla.) | 1:05.21 | Embry-Riddle First Chance Last Chance |
| 5 | APAJEE, Jessica | Tampa | 1:05.21 | USF Bulls Invitational |
| 6 | LELLIOTT, Chloe | Saint Leo | 1:07.00 | |
| 7 | WONG, Lauren | Tampa | 1:07.00 | |
| 8 | CZERWINSKI, Maria | Tampa | 1:08.00 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | BERG, Shannon | Palm Beach Atlantic | NT | |
| 2 | TAYLOR, Kirsten | Saint Leo | 1:10.90 | Embry-Riddle First Chance Last Chance |
| 3 | MARTIN, Danessa | Lynn | 1:09.00 | |
| 4 | COLLIER, Andrea | Unattached | 1:08.00 | |
| 5 | IMPAGLIAZZO, Julia | Florida Tech | 1:08.87 | Embry-Riddle First Chance Last Chance |
| 6 | PARKER, Aubrie | Lynn | 1:10.00 | |
| 7 | VATTOVAZ, Nina | Saint Leo | NT | |
| 8 | GARRETT, Anna | Saint Leo | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | MCCARTY, Maddie | Palm Beach Atlantic | NT | |
| 3 | PINDER, Lindsey | Palm Beach Atlantic | NT | |
| 4 | OSBORNE, Jessica | Warner | NT | |
| 5 | MCMAHON, Li'Mackie | Warner | NT | |
| 6 | KRUGLOVA, Jenya | Saint Leo | NT | |
| 7 | ROSADO, Fabiola | Saint Leo | NT |