| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | KOWKABANY, Ellie | Episcopal School of Jacksonville | 2:33.65 | Verified |
| 2 | GLENN, Mackenzie | Episcopal School of Jacksonville | 2:35.78 | Verified |
| 3 | BARQUILLA, Viola | Providence Middle School | 2:36.00 | Unverified: |
| 4 | SCHERER, Kaitlyn | St. Johns Classical Academy | 2:39.45 | Verified |
| 5 | ROPP, Hadley | Bolles HS | 2:41.29 | Verified |
| 6 | DRUMMOND, Kate | Oak View Middle School | 2:41.37 | Verified |
| 7 | HARDWICK, Jade | Episcopal School of Jacksonville | 2:44.20 | Unverified: |
| 8 | JOHNSTON, Brooke | Episcopal School of Jacksonville | 2:46.80 | Unverified: |
| 9 | STOUFFER, Nina | Saint Paul's Catholic School | 2:48.00 | Verified |
| 10 | ESPINO, Faith | St. Johns Classical Academy | 2:59.02 | Verified |
| 11 | CHAHLAVI, Yasmina | Bolles HS | 3:05.54 | Verified |
| 12 | BECK, Kylie | Providence Middle School | 3:06.00 | Unverified: Hand |
| 13 | RIGGSBEE, Delaney | Saint Paul's Catholic School | 3:06.16 | Verified |
| 14 | LIEB, Zoe | Bolles HS | 3:07.55 | Verified |
| 15 | LUCAS, Leila | River City Science Innovation | 3:10.80 | Verified |
| 16 | CAMPBELL, Sydney | River City Science Innovation | 3:12.45 | Verified |
| 17 | ZEHNDER, Jordan | Saint Paul's Catholic School | 3:14.79 | Verified |
| 18 | EMERY, Elliana | Bolles HS | 3:19.59 | Verified |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | HOLTON, Grace | St. Johns Classical Academy | 3:24.44 | Verified |
| 2 | SCHULTZ, Caitlynn | Providence Middle School | 3:30.12 | Verified |
| 3 | GARDERE, Elena | Saint Paul's Catholic School | 3:34.13 | Verified |
| 4 | MILLER, Charlee | Duval Charter School Baymeadows | 3:35.15 | Verified |
| 5 | BOLEN, Madison | Saint Paul's Catholic School | 3:40.00 | Unverified: |
| 6 | BURRIER, Mary | Duval Charter School Baymeadows | 3:41.57 | Verified |
| 7 | ZAEPFEL, Lydia | Bolles HS | 3:59.27 | Verified |
| 8 | OSBORNE, Savannah | Bolles HS | 4:17.03 | Verified |
| 9 | YAGZI, Tammy | Bolles HS | 4:25.55 | Verified |
| 10 | CAREY, Isabelle | Saint Paul's Catholic School | 4:30.61 | Verified |
| 11 | GRAHAM, Tai'Anah | River City Science Innovation | NT | Unverified: |
| 12 | DIXON, Rileyy | Providence Middle School | NT | Unverified: |
| 13 | DIXON, Skyy | Providence Middle School | NT | Unverified: |
| 14 | GERSTENBERGER, Gigi | Harvest Community School | NT | Unverified: |
| 15 | BALDREE, Mikalah | Beaches Chapel School | NT | Unverified: |
| 16 | JONES, Amelia | Beaches Chapel School | NT | Unverified: |
| 17 | MERINO, Ana Sofia | Duval Charter School Baymeadows | NT | Unverified: |
| 18 | WELLS, Emma | Providence Middle School | NT | Unverified: |
| 19 | HOUSE, Abigail | Episcopal School of Jacksonville | NT |