| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | RIVERA, Jenycha | Southeastern U. | 2:21.00 |
| 2 | PALM, Hannah | Southeastern U. | 2:23.00 |
| 3 | REAM, Koree | Webber | 2:31.20 |
| 4 | HIGGINS, Mariah | Southeastern U. | 2:32.00 |
| 5 | CLARK, Sharneicia | Florida Memorial | 2:34.64 |
| 6 | PALM, Ellie | Southeastern U. | 2:35.00 |
| 7 | TUCKER, Alexis | Webber | 2:35.00 |
| 8 | COPELAND, Kim | Southeastern U. | 2:45.00 |
| 9 | KURTZ, Sydney | Southeastern U. | 2:50.00 |
| 10 | CABA, Joyce | Webber | 2:52.00 |
| 11 | MENIFEE, Natasha | Webber | NT |
| 12 | CALIX, Selena | Unattached | NT |