| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 4 | Oviedo HS | A | 50.70 | Unverified: |
| 5 | Bishop Moore HS | A | NT | Unverified: |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Orlando Christian Prep | A | 45.82 | Verified |
| 2 | Hagerty HS | A | 45.52 | Verified |
| 3 | Lake Mary Prep | A | DNS | Verified |
| 4 | Winter Park HS | A | 42.10 | Verified |
| 5 | Sanford Seminole HS | A | 43.04 | Verified |
| 6 | Colonial HS | A | 43.61 | Unverified: |
| 7 | Lyman HS | A | 45.70 | Verified |
| 8 | Foundation Academy | A | 47.71 | Verified |