Top 8 times advance to finals
Finals
| Lane |
Athlete |
Team |
Seed |
| 1 |
DUDGEON, Anthony |
Lake Wales HS |
42.55 |
| 2 |
DE'JESUS, Anthony |
Lake Wales HS |
41.66 |
| 3 |
WILLIAMS, Tyson |
George Jenkins HS |
41.43 |
| 4 |
CALDWELL-FEGGINS, Joshua |
Lake Region HS |
40.43 |
| 5 |
ALLEVA, Wyland |
George Jenkins HS |
41.06 |
| 6 |
SNEED, Damarcus |
George Jenkins HS |
41.63 |
| 7 |
QUILES, Julio |
Winter Haven HS |
42.46 |
| 8 |
THOMAS, Jovaris |
Ridge Community HS |
42.69 |
Heat 1 of 4 Prelims
| Lane |
Athlete |
Team |
Seed |
| 1 |
MCGURDER, Gregory |
Bartow HS |
53.84 |
| 2 |
WOMBLE, Elijah |
Lake Region HS |
46.51 |
| 3 |
QUILES, Julio |
Winter Haven HS |
43.08 |
| 4 |
WILLIAMS, Tyson |
George Jenkins HS |
39.18 |
| 5 |
DUDGEON, Anthony |
Lake Wales HS |
42.82 |
| 6 |
WILSON, Daqwon |
Winter Haven HS |
46.13 |
| 7 |
GUTIERREZ DEL ARROYO, Nelson |
Haines City HS |
52.67 |
Heat 2 of 4 Prelims
| Lane |
Athlete |
Team |
Seed |
| 1 |
RIVERA, Jose |
Haines City HS |
54.21 |
| 2 |
ELLIOTT, George |
Lakeland Christian School |
47.74 |
| 3 |
HARVEY, Christian |
George Jenkins HS |
43.62 |
| 4 |
CALDWELL-FEGGINS, Joshua |
Lake Region HS |
40.21 |
| 5 |
THOMAS, Jovaris |
Ridge Community HS |
42.43 |
| 6 |
SWANSON, Norman |
Kathleen HS |
46.09 |
| 7 |
EASTERLINE, Tommy |
Santa Fe Catholic |
52.30 |
| 8 |
FEDDERLY, Taylor |
Santa Fe Catholic |
NT |
Heat 3 of 4 Prelims
| Lane |
Athlete |
Team |
Seed |
| 1 |
MATOS, Brandon |
Lake Wales HS |
54.51 |
| 2 |
KING, Jadarius |
Kathleen HS |
48.15 |
| 3 |
STOKES, Shyheim |
Auburndale HS |
44.02 |
| 4 |
SNEED, Damarcus |
George Jenkins HS |
40.92 |
| 5 |
CLARK, Brad |
Victory Christian Academy |
42.33 |
| 6 |
INGRAM, Brandon |
Winter Haven HS |
45.38 |
| 7 |
PAUL, Jonathon |
Haines City HS |
51.89 |
| 8 |
WHEATLEY, Antwan |
Bartow HS |
NT |
Heat 4 of 4 Prelims
| Lane |
Athlete |
Team |
Seed |
| 1 |
BUFORD, D'Shawn |
Lakeland HS |
56.12 |
| 2 |
BEST, Carlos |
Lakeland HS |
48.64 |
| 3 |
FRASER, Isaiah |
Ridge Community HS |
44.26 |
| 4 |
ALLEVA, Wyland |
George Jenkins HS |
41.82 |
| 5 |
DE'JESUS, Anthony |
Lake Wales HS |
42.28 |
| 6 |
KORNBRUST, Joe |
Lakeland Christian School |
44.30 |
| 7 |
NESTER, Austin |
McKeel Academy |
48.91 |
| 8 |
ALKASS, Andrew |
Santa Fe Catholic |
57.14 |
