| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Allen, Kerrin | SR | East River HS | NM | Unverified: |
| 2 | Stelljes, Reagan | 9 | Apopka HS | 1.12m | Verified |
| 3 | Warren, Jarkevia | SO | Lake Mary HS | 1.12m | Verified |
| 4 | Gonzalez, Trinity | 9 | Lake Mary HS | 1.15m | Verified |
| 5 | Garnett, Jayden | JR | Lake Mary HS | 1.27m | Verified |
| 6 | Graham, Mysha | SO | Lake Wales HS | 1.28m | Verified |
| 7 | Quezada, Emily | SO | Lake Mary HS | 1.32m | Verified |
| 8 | Sanders, Brindesi | SO | Lake Nona HS | 1.37m | Verified |
| 9 | Cruz, Angeliz | SO | Wekiva HS | 1.37m | Verified |
| 10 | Ayala, Charlette | SO | Lake Nona HS | 1.40m | Verified |
| 11 | Manfredi, Valerie | JR | Orange City University HS | 1.42m | Verified |
| 12 | Butler, Jordan | JR | Lake Howell HS | 1.47m | Verified |
| 13 | Long, Rachel | SO | Wekiva HS | 1.47m | Verified |
| 14 | Towle, Stephanie | SO | Lake Nona HS | 1.47m | Verified |
| 15 | Hillery, Zarria | SR | Orange City University HS | 1.47m | Verified |
| 16 | Lino, Lazaria | SR | Evans HS | 1.62m | Verified |