| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Thomas, Lauren | SR | Orange City University HS | 1:03.81 | Verified |
| 2 | Clelland-Goddard, Kiona | JR | Lyman HS | 1:03.31 | Unverified: |
| 3 | Palelis, Sarah | SR | Orange City University HS | 1:02.86 | Verified |
| 4 | Joseph, Jasmyne | SR | Evans HS | 57.89 | Verified |
| 5 | Garcia, Leslie | SR | Lyman HS | 1:00.73 | Verified |
| 6 | Labrato, Christina | 9 | Lake Mary HS | 1:03.15 | Verified |
| 7 | Miles, Shakiena | SR | Lake Mary HS | 1:03.80 | Verified |
| 8 | Butler, Iysha | SR | Lake Mary HS | 1:03.87 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Leake, Madison | SO | Orange City University HS | 1:05.70 | Verified |
| 2 | Towle, Stephanie | SO | Lake Nona HS | 1:05.00 | Unverified: |
| 3 | Long, Rachel | SO | Wekiva HS | 1:04.84 | Verified |
| 4 | Smith, Nicole | SR | Sanford Seminole HS | 1:04.00 | Verified |
| 5 | Graham, Mysha | SO | Lake Wales HS | 1:04.61 | Verified |
| 6 | Laurore, Keturah | SO | Wekiva HS | 1:04.90 | Verified |
| 7 | Gordon, Madison | 9 | Lyman HS | 1:05.62 | Verified |
| 8 | Mccalla-Clark, Daimone | JR | Sanford Seminole HS | 1:06.00 | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Faulkner, Kayla | SO | Lake Mary HS | 1:08.33 | Verified |
| 2 | Reese, Kennedie | SR | Lake Mary HS | 1:07.26 | Verified |
| 3 | Gil, Andrea | SR | Lake Nona HS | 1:07.00 | Unverified: |
| 4 | Fye, Zaniyah | 9 | Sanford Seminole HS | 1:06.11 | Verified |
| 5 | Berry, Eldria | JR | Lyman HS | 1:06.51 | Verified |
| 6 | Quezada, Emily | SO | Lake Mary HS | 1:07.24 | Verified |
| 7 | Figuero, Anna | JR | Sanford Seminole HS | 1:07.99 | Verified |
| 8 | Cruz, Angeliz | SO | Wekiva HS | 1:10.10 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Estrella, Natalie | JR | Sanford Seminole HS | 1:14.46 | Verified |
| 2 | Feagin, Samya | SO | Lake Wales HS | 1:12.73 | Verified |
| 3 | McClellan, Jessica | SO | Hagerty HS | 1:12.01 | Verified |
| 4 | Bradley, Cymiah | SO | Lake Mary HS | 1:11.33 | Verified |
| 5 | Turner, Tamia | 9 | Lake Mary HS | 1:11.71 | Verified |
| 6 | Toro, Cailyn | JR | Orange City University HS | 1:12.73 | Verified |
| 7 | Thomas, Cameryn | SO | Lake Mary HS | 1:13.20 | Verified |
| 8 | Gagliardi, Emily | 9 | Lake Mary HS | 1:16.30 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Diaz, Ana | 9 | Lake Mary HS | NT | Unverified: |
| 2 | Matthews, Laniyah | 9 | Halifax Academy | NT | Unverified: |
| 3 | Benitez-Zayas, Maria | SO | Lake Mary HS | NT | Unverified: |
| 4 | Cummings, Katherine | 9 | Lake Mary HS | 1:32.90 | Verified |
| 5 | Lauderdale, Amaya | SO | Lake Wales HS | NT | Unverified: |
| 6 | Moore, Alexis | SO | Halifax Academy | NT | Unverified: |
| 7 | Scarvers, Eniyah | JR | Lake Wales HS | NT | Unverified: |
| 8 | Young, Amiyah | 6 | Halifax Academy | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Arroyo, Lesliany | SO | Hagerty HS | NT | Unverified: |
| 2 | Morales, Isabel | JR | Lake Howell HS | NT | Unverified: |
| 3 | Powell, Jessiah | SO | Lake Mary HS | NT | Unverified: |
| 4 | Champlin, Bethany | 9 | Hagerty HS | NT | Unverified: |
| 5 | Cherugail, Roshna | 9 | Hagerty HS | NT | Unverified: |
| 6 | Lee, Julissa | 9 | Lake Mary HS | NT | Unverified: |
| 7 | Johnson, Autumn | JR | Halifax Academy | NT | Unverified: |
| 8 | Bradley, Cheyenne | 9 | Lake Mary HS | NT | Unverified: |