| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | JEAN, Avrel | HB Plant HS | 27.62 |
| 2 | GADSON, Serena | George M. Steinbrenner HS | 26.02 |
| 3 | GADSON, Ken'naria | George M. Steinbrenner HS | 26.31 |
| 4 | HARGROVE, LaSarah | Gaither HS | 24.82 |
| 5 | BENJAMIN, Shaniya | Alonso HS | 25.27 |
| 6 | JACK, Zayne | George M. Steinbrenner HS | 26.91 |
| 7 | BROOKS, Hannah | George M. Steinbrenner HS | 27.37 |
| 8 | SINGLETON, Maya | Alonso HS | 28.19 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | LARK, denajae | Tampa Jefferson HS | 32.00 | Unverified: |
| 2 | LALOR, Angel | Howard W. Blake HS | 29.39 | Verified |
| 3 | CAMPBELL, Chelsey | Robinson HS | 26.88 | Verified |
| 4 | HARGROVE, LaSarah | Gaither HS | 23.59 | Verified |
| 5 | BROOKS, Hannah | George M. Steinbrenner HS | 26.83 | Verified |
| 6 | THOMAS, Janessa | Alonso HS | 28.36 | Verified |
| 7 | TAYLOR, Jala | Sickles HS | 29.73 | Verified |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | PHILYOR, Eliayah | Tampa Jefferson HS | 32.04 | Verified |
| 2 | SHELTON, Morgan | HB Plant HS | 29.42 | Verified |
| 3 | HAFFNER, Alyssa | Sickles HS | 28.03 | Verified |
| 4 | BENJAMIN, Shaniya | Alonso HS | 24.38 | Verified |
| 5 | GADSON, Serena | George M. Steinbrenner HS | 25.27 | Verified |
| 6 | JAMES, Avriel | Robinson HS | 29.00 | Unverified: |
| 7 | CARRINGTON-JOSEPH, Nubia | Howard W. Blake HS | 31.68 | Verified |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | VARNES, Stephanie | Gaither HS | 39.04 | Verified |
| 2 | FIERRO, Jezel | Sickles HS | 29.47 | Verified |
| 3 | SINGLETON, Maya | Alonso HS | 27.18 | Verified |
| 4 | JACK, Zayne | George M. Steinbrenner HS | 24.81 | Verified |
| 5 | COLLADO, Mia | HB Plant HS | 26.11 | Verified |
| 6 | RAMSEY, Jada | Robinson HS | 28.96 | Verified |
| 7 | THOMAS, Tiana | Howard W. Blake HS | 31.20 | Verified |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | SANDLIN, Imari | Howard W. Blake HS | 32.57 | Verified |
| 2 | SEABURY, Essence | Gaither HS | 29.56 | Unverified: |
| 3 | CORDOBA, Jarlenys | Alonso HS | 27.76 | Verified |
| 4 | GADSON, Ken'naria | George M. Steinbrenner HS | 24.90 | Verified |
| 5 | JEAN, Avrel | HB Plant HS | 25.92 | Verified |
| 6 | GARCIA-HECTOR, Naomi | Sickles HS | 28.55 | Verified |
| 7 | HARRELL, Jasmine | Robinson HS | 29.56 | Verified |
| 8 | REILEY, Khalilah | Tampa Jefferson HS | NT | Unverified: |