| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Leto HS | A | NT | Unverified: |
| 2 | Robinson HS | A | 3:49.27 | Verified |
| 3 | Alonso HS | A | 3:32.15 | Verified |
| 4 | Tampa Jefferson HS | A | 3:28.57 | Verified |
| 5 | George M. Steinbrenner HS | A | 3:28.79 | Verified |
| 6 | Sickles HS | A | 3:39.72 | Verified |
| 7 | Gaither HS | A | 3:52.25 | Verified |
| 8 | HB Plant HS | A | NT | Unverified: |