| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | PALETTI, Jonathan | Dixie Hollins HS | 44.21 | Verified |
| 2 | PRESCOTT, Royce | Countryside HS | 43.76 | Verified |
| 3 | CLEPPE, Shaun | Osceola HS (Seminole) | 42.75 | Verified |
| 4 | BERNITT, Donovan | Countryside HS | 41.07 | Verified |
| 5 | CASTLE, Jake | East Lake HS | 41.87 | Verified |
| 6 | BETHUNE, Christopher | St. Petersburg HS | 43.32 | Verified |
| 7 | HARRIS, Alonzo | Lakewood HS | 43.87 | Verified |
| 8 | FANGER, Jakob | Dunedin HS | 44.38 | Verified |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | EVANS, Keyon | Gibbs HS | 46.71 | Verified |
| 2 | CRESSWELL, Richard | Osceola HS (Seminole) | 46.50 | Verified |
| 3 | BLANDING, Sean | Osceola HS (Seminole) | 45.89 | Verified |
| 4 | MOISE, Jonathon | Lakewood HS | 45.11 | Verified |
| 5 | MEADOWS, Noah | Palm Harbor University HS | 45.74 | Verified |
| 6 | RANDOLPH, Reginald | Northeast HS (St. Petersburg) | 45.95 | Verified |
| 7 | SUTHERLAND, Jae | St. Petersburg HS | 46.54 | Verified |
| 8 | BAILEY, Hunter | Osceola HS (Seminole) | 46.71 | Verified |