| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | BIGGS, Dalayna | Lake Region HS | 53.25 |
| 2 | GONZALEZ, Ashley | George Jenkins HS | 52.38 |
| 3 | BARR, Heaven | George Jenkins HS | 51.05 |
| 4 | CLARKE, Umayah | Kathleen HS | 49.75 |
| 5 | MASON, Caroline | George Jenkins HS | 50.26 |
| 6 | JAMES, Ja'Niya | Kathleen HS | 52.11 |
| 7 | FOSTER, Alexis | George Jenkins HS | 52.71 |
| 8 | MOORE, jasantis | Lake Region HS | 54.09 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 3 | OSORIO, Emely | George Jenkins HS | 1:00.68 |
| 4 | PREVATT, Brooke | Victory Christian Academy | 58.61 |
| 5 | FORMAN, Kirsten | Victory Christian Academy | 59.19 |
| 6 | GOODING, Hallie | George Jenkins HS | 1:05.12 |