| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | JENKINS, Kaylee | George Jenkins HS | 28.36 |
| 2 | DOCTOR, Diamond | Kathleen HS | 27.07 |
| 3 | THOMPSON, Chamere | George Jenkins HS | 26.22 |
| 4 | WILSON, Zy'Eisha | Kathleen HS | 24.84 |
| 5 | WILSON, I'Asia | Kathleen HS | 25.25 |
| 6 | DMYTRYSZYN, Victoria | George Jenkins HS | 26.84 |
| 7 | RUIZ, Alicia | George Jenkins HS | 28.00 |
| 8 | WRIGHT, Dazarae | Kathleen HS | 28.76 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | CHANCEY, Madison | George Jenkins HS | 31.70 |
| 2 | STANALAND, Jennifer | George Jenkins HS | 30.50 |
| 3 | PEETS, Alia | George Jenkins HS | 30.31 |
| 4 | PITT, Khalan | Victory Christian Academy | 29.70 |
| 5 | WRIGHT, Jalayla | George Jenkins HS | 30.26 |
| 6 | LAKE, Jessica | George Jenkins HS | 30.31 |
| 7 | JORDAN, Nahyah | Lake Region HS | 31.08 |
| 8 | LEE, Cameron | George Jenkins HS | 31.93 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | WHITE, Kim | George Jenkins HS | 36.33 |
| 2 | CLEMENT, Hannah | Victory Christian Academy | 33.33 |
| 3 | HURD, D'Asia | George Jenkins HS | 32.65 |
| 4 | MCGOUGH, Jaila | Lake Region HS | 32.01 |
| 5 | GONZALEZ DIAZ, Liahneli | Victory Christian Academy | 32.34 |
| 6 | ORTIZ PARRA, Angela | George Jenkins HS | 33.05 |
| 7 | MORENO, Asdrid | George Jenkins HS | 33.84 |
| 8 | RODRIGUEZ, Jossy | Victory Christian Academy | NT |