| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 3 | Winter Springs HS | A | NT | Unverified: |
| 4 | Lake Howell HS | A | DNF | Unverified: |
| 5 | Crooms Academy HS | A | NT | Unverified: |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | Hagerty HS | A | 44.90 | Verified |
| 3 | Lake Mary HS | A | 43.59 | Verified |
| 4 | Sanford Seminole HS | A | 42.75 | Verified |
| 5 | Lake Brantley HS | A | 43.40 | Verified |
| 6 | Lyman HS | A | 44.60 | Verified |
| 7 | Oviedo HS | A | 46.84 | Verified |