| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BELL, Alyssa | 9 | Orange City University HS | 57.59 | Verified |
| 2 | APONTE, Aleja | 9 | Space Coast HS | 57.16 | Verified |
| 3 | HUBBERT, Asia | JR | Mainland HS | 52.99 | Verified |
| 4 | COOPER, Jourdan | SO | Mainland HS | 51.61 | Verified |
| 5 | WUERFFEL, Audrey | 9 | Harvest Community School | 52.90 | Verified |
| 6 | KLEIN, Summer | SO | Mainland HS | 54.85 | Verified |
| 7 | MARTIN, Briahna | 9 | Orange City University HS | 57.58 | Verified |
| 8 | FERNANDEZ, Samantha | SR | St. Brendan HS | 59.47 | Verified |