| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | ESPANA, Marcos | SR | Osceola HS (Kissimmee) | 44.31 | Verified |
| 3 | AMADIS, Gabriel | JR | Osceola HS (Kissimmee) | 42.79 | Verified |
| 4 | WILLIAM, Jeremiah | SR | Osceola HS (Kissimmee) | 40.98 | Verified |
| 5 | COOPER, Lindon | JR | Mainland HS | 41.35 | Verified |
| 6 | ELLIS, Raheem | SR | Mainland HS | 43.64 | Verified |
| 7 | MATHIS, Isaiah | SR | Osceola HS (Kissimmee) | 44.85 | Verified |
| 8 | HALAVIN, Noah | SR | Space Coast HS | 47.07 | Verified |