| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Mainland HS | A | Reed, Malcolm | Cooper, Lindon | Jenkins, Brian | Kamara, Richard | DNS | Verified |
| 2 | Mainland HS | B | Collins, Arnold | Davis, Quenton | Reilly, Jonah | Guillory, Andrew | DNS | Verified |
| 3 | Osceola HS (Kissimmee) | A | 9:24.28 | Verified | ||||
| 4 | Space Coast HS | A | Campos, Sean | Davis, Stephen | Sanchez, Declan | Russel, Ethan | 9:54.55 | Verified |