| Lane | Team | Squad | Seed |
|---|---|---|---|
| 3 | East River HS | A | 45.56 |
| 4 | University HS | A | 44.66 |
| 5 | Lake Nona HS | A | 44.82 |
| Lane | Team | Squad | Seed |
|---|---|---|---|
| 2 | Oak Ridge HS | A | 44.31 |
| 3 | Boone HS | A | 43.58 |
| 4 | Winter Park HS | A | 42.10 |
| 5 | Freedom HS (Orlando) | A | 42.47 |
| 6 | Timber Creek HS | A | 43.85 |
| 7 | Colonial HS | A | 44.57 |