| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | DRUMMOND, Kate | 7 | Oak View Middle School | 5:38.90 | Verified |
| 2 | BATA, Leila | 6 | Bolles HS | 5:39.04 | Verified |
| 3 | GLENN, Mackenzie | 8 | Episcopal School of Jacksonville | 5:41.95 | Verified |
| 4 | BARQUILLA, Viola | 8 | Providence Middle School | 5:43.55 | Verified |
| 5 | WYATT, Alyssa | 8 | Duval Charter School Baymeadows | 5:48.98 | Verified |
| 6 | KOWKABANY, Hannah | 6 | Episcopal School of Jacksonville | 5:55.14 | Verified |
| 7 | COWART, Adalie | 8 | Suwannee Middle School | 6:02.09 | Verified |
| 8 | SYKORA, Gigi | 6 | Saint Paul's Catholic School | 6:05.85 | Verified |
| 9 | SIMPSON, Sarah | 7 | Lake City Middle School | 6:07.98 | Verified |
| 10 | WOODS, Sydney | 7 | Providence Middle School | 6:08.85 | Unverified: UNF Pre-state |
| 11 | WELLS, Emma | 6 | Providence Middle School | 6:13.58 | Verified |
| 12 | SNYDER, Megan | 6 | St. Elizabeth Ann Seton Catholic | 6:14.90 | Verified |
| 13 | FENDER, Audrey | 7 | Lake City Middle School | 6:23.08 | Verified |
| 14 | MUNDIN, Anne Harvey | 8 | Bolles HS | 6:30.00 | Unverified: |
| 15 | MUNDIN, Townsend | 7 | Bolles HS | 6:30.00 | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | GERSTENBERGER, Gigi | 8 | Harvest Community School | 6:32.68 | Verified |
| 2 | SAMS, Ava | 7 | Blessed Trinity Catholic School | 6:35.75 | Verified |
| 3 | GARCES, Catherine | 5 | St. Elizabeth Ann Seton Catholic | 6:52.14 | Verified |
| 4 | SCHULER, Abigail | 7 | Lake City Middle School | 7:06.72 | Verified |
| 5 | ANDREWS, Naylim | 5 | Blessed Trinity Catholic School | 7:08.22 | Verified |
| 6 | HAYNES, Amelia | 8 | Episcopal School of Jacksonville | 7:17.31 | Verified |
| 7 | CHENG, Audrey | 8 | Bolles HS | 7:17.32 | Verified |
| 8 | ALVES-SILVA, Leticia | 5 | Blessed Trinity Catholic School | 7:18.70 | Verified |
| 9 | HOENI, Addison | 5 | St. Elizabeth Ann Seton Catholic | 7:19.48 | Verified |
| 10 | CLANCY, Cailey | 5 | Saint Paul's Catholic School | 7:19.79 | Verified |
| 11 | TAVARES, Carina | 7 | PAL Indian Trails Middle School | 7:39.29 | Verified |
| 12 | HERNANDEZ, Valeria | 5 | Blessed Trinity Catholic School | 7:42.54 | Verified |
| 13 | JOLICEOUR, Lucy | 7 | Suwannee Middle School | 8:09.27 | Verified |
| 14 | WODCHIS, Sophya | 7 | PAL Indian Trails Middle School | NT | Unverified: |