| Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note | |
|---|---|---|---|---|---|---|---|---|
| 1 | Wiregrass Ranch | A | Medina, Alejandro | Morales Ramos, Alex | Alvarez, Emanuel | Dimarco, Anthony | 8:30.00 | |
| 2 | George Steinbrenner | A | Foreman, Paul | Harrigan, Zachary | Moorer, Kaleb | Whitmer, Benjamin | 8:32.00 | |
| 3 | Sickles | A | McKenna, Tyler | Newton, Jahbari | Stavrou, Alex | Cummings, Andre | 8:34.00 | Charles Johnson Jr. Invitational |
| 4 | Chamberlain | A | Davis, Jockyll | Fogg, Harrison | Vu, Chi | Velasquez, Ernesto | 8:36.58 | |
| 5 | Gaither | A | Korloch, Christopher | Prozer, Austin | Quesada, Alex | Summers, Tyler | 8:42.09 | FHSAA Class 3A-District 9 |
| 6 | Middleton | A | Evina-Ze, Alexis | Allen, Tico | Jones, Mike | Jackson, Anthony | 8:54.36 | FHSAA 2A Region 3 |
| 7 | Armwood | A | Aponte, Luis | Bryant, Lamar | Star, Camari | Brown, Sean | 9:03.39 | |
| 8 | Freedom (Tampa) | A | Fuhrmeister, Ethan | Benitez, Andre | Cory, Andrew | Smith, Owen | 9:32.00 | |
| 9 | Carrollwood | A | Casey, Carter | Olson, Eric | Colbath, Gabriel | Blazowski, Evan | 9:50.55 |