| Athlete | Yr. | Team | Seed | Note | |
|---|---|---|---|---|---|
| 1 | Daniel, Leah | SR | King | 25.56 | |
| 2 | Jones, Ajah | SR | Wharton | 26.20 | |
| 3 | Haag, Sophie | 8 | Carrollwood | 26.55 | |
| 4 | Ransom, Sharielle | JR | Tampa Bay Tech | 26.58 | |
| 5 | Apple, Riley | JR | Gaither | 27.20 | |
| 6 | Gosa, Kailah | JR | Tampa Bay Tech | 27.82 | |
| 7 | Johnson, Tyjai | JR | Tampa Bay Tech | 27.85 | |
| 8 | Takeli, Amsy | SO | Tampa Bay Tech | 28.00 | |
| 9 | Garanton, Camila | FR | Carrollwood | 28.02 | FHSAA 1A Region 2 |
| 10 | Bainbridge, Stephanie | SO | Carrollwood | 28.17 | |
| 11 | Myers, Clinesha | JR | Chamberlain | 28.20 | |
| 12 | Durr, Ciarra | FR | King | 28.20 | |
| 13 | Woeste, Matheson | SO | Carrollwood | 28.45 | |
| 14 | Brown, Christian | SR | King | 28.50 | |
| 15 | Burford, Kortney | JR | Wharton | 28.51 | |
| 16 | Simmons, Janiyah | JR | King | 28.60 | |
| 17 | Bell, Durnecia | FR | Hillsborough | 28.60 | |
| 18 | Fierro, Jezel | SR | Sickles | 28.63 | |
| 19 | Webb, Azriel | FR | Hillsborough | 28.90 | |
| 20 | Day, Erykah | SO | Wharton | 29.11 | |
| 21 | Morris , Quewana | SO | Middleton | 29.60 | |
| 22 | Tedeschi, Angelina | SO | Wharton | 29.80 | |
| 23 | Andujar, Jamaist | SR | Chamberlain | 29.87 | |
| 24 | Smalls, Jasmine | SO | Chamberlain | NT | |
| 25 | Cooper, Taliyah | FR | Chamberlain | NT | |
| 26 | Limehouse, J'Mi | JR | Armwood | NT | |
| 27 | Love, Gariona | SO | Armwood | NT | |
| 28 | Wells, Ani | JR | Armwood | NT | |
| 29 | Buggs, Lauren | FR | Sickles | NT | |
| 30 | Jaime, Emely | SO | Sickles | NT | |
| 31 | Gainey, Genesis | JR | Sickles | NT | |
| 32 | Underwood, Raejean | FR | Middleton | NT | |
| 33 | Massilion, Sarah | FR | Middleton | NT | |
| 34 | Massillon, Sinia | SR | Middleton | NT | |
| 35 | Yepez, Isabella | FR | Holy Names | NT | |
| 36 | Merriman, Victoria | FR | Holy Names | NT | |
| 37 | Gonzalez, Catalina | FR | Holy Names | NT | |
| 38 | Rolack, Christal | FR | Robinson | NT | |
| 39 | Schoonmaker, Shelby | SO | Robinson | NT | |
| 40 | James, Avriel | JR | Robinson | NT | |
| 41 | Faddoul, Christy | JR | Wiregrass Ranch | NT | |
| 42 | Brown, Laila | SR | Wiregrass Ranch | NT | |
| 43 | Lowther, Qyana | JR | Wiregrass Ranch | NT | |
| 44 | Finkle, Shaina | FR | Wiregrass Ranch | NT | |
| 45 | Bartley, Honesty | FR | Lake Gibson | NT | |
| 46 | Small , Calli Ann | SR | Lake Gibson | NT | |
| 47 | Alexander, J'lae | FR | Lake Gibson | NT | |
| 48 | Underwood, Carlaysia | FR | Lake Gibson | NT | |
| 49 | Poindexter, Amara | JR | Hillsborough | NT | |
| 50 | Higginbotham, Karissa | SR | Hillsborough | NT | |
| 51 | Hicks, MacKenzie | JR | Tampa Catholic | NT | |
| 52 | Resiliac, Bedjena | SO | Tampa Catholic | NT |