| Athlete | Yr. | Team | Seed | Note | |
|---|---|---|---|---|---|
| 1 | Lydon, Elizabeth | SO | Gaither | 59.90 | |
| 2 | Murphy, Justyce | FR | Hillsborough | 1:00.23 | |
| 3 | Ransom, Sharielle | JR | Tampa Bay Tech | 1:01.24 | FHSAA Class 3A-District 9 |
| 4 | Tate, Lauryn | JR | Tampa Catholic | 1:02.10 | |
| 5 | Bainbridge, Stephanie | SO | Carrollwood | 1:02.92 | FHSAA 1A Region 2 |
| 6 | Leepack, Molly | JR | Holy Names | 1:02.96 | |
| 7 | Matos, Jeanlee | SR | Freedom (Tampa) | 1:03.00 | |
| 8 | Morris , Quewana | SO | Middleton | 1:03.00 | |
| 9 | Hicks, MacKenzie | JR | Tampa Catholic | 1:04.00 | |
| 10 | Kerr, Natasha | SR | Tampa Bay Tech | 1:05.46 | FHSAA Class 3A-District 9 |
| 11 | Webb, Azriel | FR | Hillsborough | 1:05.60 | |
| 12 | Woodall, Shawna | SO | Hillsborough | 1:06.20 | |
| 13 | Gutcher, Carley | SO | Gaither | 1:07.00 | |
| 14 | Bell, Durnecia | FR | Hillsborough | 1:07.40 | |
| 15 | McGuane, Margaret | SO | Holy Names | 1:09.49 | |
| 16 | Bridgewater, Chelsi | JR | Wharton | 1:10.00 | |
| 17 | Martiner, Emily | SO | Carrollwood | 1:10.00 | |
| 18 | Jaime, Emely | SO | Sickles | 1:12.00 | |
| 19 | Andujar, Jamaist | SR | Chamberlain | 1:12.63 | |
| 20 | Myers, Clinesha | JR | Chamberlain | NT | |
| 21 | Cooper, Taliyah | FR | Chamberlain | NT | |
| 22 | Limehouse, J'Mi | JR | Armwood | NT | |
| 23 | Gadson, Kennaria | JR | Wharton | NT | |
| 24 | Lavine, Fallon | SO | Wharton | NT | |
| 25 | Jones, Naomi | FR | Wharton | NT | |
| 26 | Shelton, Natalie | SR | Sickles | NT | |
| 27 | Buggs, Lauren | FR | Sickles | NT | |
| 28 | Fierro, Jezel | SR | Sickles | NT | |
| 29 | Rolack, Christal | FR | Robinson | NT | |
| 30 | Richardson, Jalyssa | SR | Robinson | NT | |
| 31 | Schoonmaker, Shelby | SO | Robinson | NT | |
| 32 | Williams, Dahlia | FR | Wiregrass Ranch | NT | |
| 33 | Hylton, Norr | SR | Wiregrass Ranch | NT | |
| 34 | Khalil, Maryam | FR | Wiregrass Ranch | NT | |
| 35 | Thompson, Afiya | SO | Wiregrass Ranch | NT | |
| 36 | Bennett, Rachel | FR | Lake Gibson | NT | |
| 37 | Underwood, Carlaysia | FR | Lake Gibson | NT | |
| 38 | Morrison, Veloria | FR | Lake Gibson | NT |