| Athlete | Yr. | Team | Seed | Note | |
|---|---|---|---|---|---|
| 1 | Otero, Nicolina | SR | Wharton | 2:20.00 | |
| 2 | Zavala, Lycia | SR | Hillsborough | 2:22.56 | FHSAA Class 3A-District 9 |
| 3 | Gordon, Ciana | SO | Armwood | 2:27.88 | FHSAA Class 3A-District 9 |
| 4 | Reif, Brooke | FR | Wharton | 2:33.00 | |
| 5 | Fredericks, Alexa | JR | Holy Names | 2:33.57 | |
| 6 | HIll, Mya | SO | Wiregrass Ranch | 2:34.00 | |
| 7 | Johnson, Jinia | JR | Wharton | 2:35.00 | |
| 8 | Warren, Kourtney | SO | King | 2:35.25 | |
| 9 | Stoltzfoos, Sydney | SR | Gaither | 2:38.00 | |
| 10 | Abrahamsen, Madelynn | SR | Wiregrass Ranch | 2:38.00 | |
| 11 | Jayaraj, Michelle | JR | King | 2:38.50 | |
| 12 | Moore, Kate | JR | Wiregrass Ranch | 2:41.00 | |
| 13 | Detjen, Skylar | SR | King | 2:45.00 | |
| 14 | Cabral, Nina | SR | Sickles | 2:50.00 | |
| 15 | Kompella, Amulya | FR | King | 2:53.00 | |
| 16 | Severy, Madeline | FR | Wiregrass Ranch | 2:56.00 | |
| 17 | JOHNSON, ALEXCIS | SO | Hillsborough | 2:59.00 | |
| 18 | Sarsenova, Aida | FR | Gaither | 3:10.00 | |
| 19 | Fernandez, Natasha | SO | Gaither | 3:15.00 | |
| 20 | Thompson, Constane | FR | Middleton | 3:50.00 | |
| 21 | Sanchez, Yasmin | FR | Chamberlain | NT | |
| 22 | Cruz, Angelina | SR | Chamberlain | NT | |
| 23 | Jones, Maliah | SR | Armwood | NT | |
| 24 | Flake, Brianna | SO | Armwood | NT | |
| 25 | Perez, Jessica | JR | Wharton | NT | |
| 26 | King, Mariah | SO | Tampa Bay Tech | NT | |
| 27 | Molina, Jailene | JR | Sickles | NT | |
| 28 | Hartman, Sommer | SO | Sickles | NT | |
| 29 | Acostandei, Nicole | SO | Sickles | NT | |
| 30 | Andrews, Sole | SR | Hillsborough | NT | |
| 31 | Lam, Mandy | SO | Tampa Catholic | NT |