| Lane | Athlete | Team | Note |
|---|---|---|---|
| 1 | DAVIS, Jeanne | Oviedo | FHSAA Class 4A-District 2 |
| 2 | ZAPATA, Isabela | Lake Howell | |
| 3 | SURESH, Varsha | Oviedo | |
| 4 | DAVIS, Joyce | Oviedo | |
| 5 | QIAO, Amy | Trinity Prep | |
| 6 | KEEFE, Leah | Trinity Prep | |
| 7 | PARENT, Meg | Trinity Prep | |
| 8 | CULLISON, Olivia | Oviedo | |
| 9 | DAVEY, Emma | Trinity Prep | |
| 10 | DRISCOLL, Elizabeth | Trinity Prep | |
| 11 | BIGNEY, Jaiden | Trinity Prep | |
| 12 | KORTMAN, Ava | Trinity Prep | |
| 13 | AUNE, Amalie | Oviedo | |
| 14 | WILKEN-YODER, Kathe | Hagerty | |
| 15 | ECHAVARRIA, Jorgelina | Trinity Prep | |
| 16 | GOMEZ, Katie | Lake Howell | |
| 17 | KIMBALL, Kyra | Hagerty | |
| 18 | FRUHWIRTH, Sydney | Oviedo | |
| 19 | PROSTAK, Madalyn | Lake Howell | |
| 20 | HOMANN, Sina | Hagerty | |
| 21 | THOMPSON, Charissa | Hagerty |