| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Cypress Lake | A | Dorisme, Kimberline | Dulin, Kennedy | Thompson, Taylor | Lawrence, Alisha | DNS | |
| 2 | Bishop Verot | A | Lewis, Analise | Sarnac, Ella | Muller, Samantha | Shahriari, Sophia | 50.17 | 2018 FHSAA State Track & Field Championships |
| 3 | Dunbar | A | Stewart, Teera | Weaver, Lucheyona | Hardwick, Melanie | Soloma, Gabriella | 49.12 | 2018 FHSAA State Track & Field Championships |
| 4 | First Baptist (Naples) | A | Beam, Sydney | Munn, Gena | Lines, Amanda | L, Annika | 49.93 | 2018 FHSAA State Track & Field Championships |
| 5 | Fort Myers | A | LaFosse, Benjelle | Victor, Jaziah | Talbert, Idalia | Corgelas, Brittney | DNS | |
| 6 | St. John Neumann | A | Bergstrom, Natalie | De Armas, Ashley | Jean-Felix, Bianca | Martin-Gonzales, Leah | 52.50 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Village School | A | Stuben, Brooklyn | Olson, Eleanor | Sweet, Samantha | Walker, Annaleigh | 58.20 | |
| 2 | Moore Haven | A | Ling, Akeelah | Dunkley, Arifaith | Williams, Kaleigh | Thomas, Adore | 54.45 | |
| 3 | Riverdale | A | Hardy, Ma'kerra | Frison, Mahogany | Saddlar, Shontae | Kendrick, Torrey | 52.84 | Ida Baker FD Indoor/Outdoor Invite |
| 4 | Canterbury (FM) | A | Miller, Ellie | Reeves, Erin | Haber, Anna | Bradford, Morgan | 53.21 | FHSAA Class 1A-District 8 |
| 5 | Immokalee | A | Deceus, Ashley | Vilcin, Kathiana | Zamor, Calouna | Hollins , Malaya | 56.97 | FHSAA Class 2A-District 12 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Island Coast | A | Carter, Alliyah | Vendredy, Beverly | Pierre, Kicha | Smith, Petagaye | NT | |
| 3 | Cape Coral | A | Altidor, Grace | Dumond, Samara | Fajardo, Jazmine | Rodriguez, Jalis | 58.40 | |
| 4 | Evangelical Christian | A | Appell, Marlee | Valentine, Claire | Masso, Manon | Bereczki, Alexa | 1:03.00 |