| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | DeLand | A | 3:37.44 | DeLand/Deltona Meet #1 |
| 2 | Lake Minneola | A | 3:42.22 | Carl Friedland Memorial 2019 |
| 3 | Celebration | A | 3:34.00 | |
| 4 | Mainland | A | 3:23.00 | |
| 5 | Winter Park | A | 3:30.00 | |
| 6 | Evans | A | 3:35.00 | |
| 7 | Boone | A | 3:35.22 | Boone HS Quad 2019 |
| 8 | Wekiva | A | 3:40.00 |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 3 | Timber Creek | A | 3:44.48 | Highlander Invitational 2019 |
| 4 | Colonial | A | 3:37.83 | Saint Cloud Invitational 2019 |
| 5 | East River | A | 3:41.55 | Satellite High Invitational |
| 6 | Lake Howell | A | 3:55.00 | |
| 7 | Edgewater | A | 3:41.00 |