| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Celebration | A | 4:23.70 | Storm Preview |
| 2 | Wekiva | A | NT | |
| 3 | Evans | A | NT | |
| 4 | Jones | A | 4:04.37 | Carl Friedland Memorial 2019 |
| 5 | Mainland | A | 4:10.00 | |
| 6 | Timber Creek | A | 4:15.00 | |
| 7 | Lake Minneola | A | 4:22.94 | Carl Friedland Memorial 2019 |
| 8 | DeLand | A | 4:28.07 | East Coast Classic |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Colonial | A | 4:31.40 | Sanford Seminole Open at the NTC |
| 2 | Orlando Christian Prep | A | DNS | |
| 3 | Lake Howell | A | 4:38.21 | Lake Howell QUAD 2019 |
| 4 | Boone | A | 4:31.97 | Boone HS Quad 2019 |
| 5 | East River | A | 4:41.45 | FHSAA 3A Region 2 |
| 6 | Winter Park | A | 4:40.00 |