| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 191 | URBINA, Juleigh | 7 | McKeel | 1:12.15 | Lake Region Open |
| 2 | 95 | CONLIFFE, Lakeisha | FR | Auburndale | 1:09.62 | Lake Region Open |
| 3 | 94 | JOHNSON, Riniya | JR | Auburndale | 1:03.56 | George Jenkins Invitational |
| 4 | 39 | JAMES, April | SR | Lake Region | 1:02.57 | Lake Region Open |
| 5 | 38 | PIERRE-LOUIS, Wislande | SR | Lake Region | 1:02.71 | George Jenkins Invitational |
| 6 | 154 | NEWLAND, Savanna | 8 | McKeel | 1:05.61 | Lake Region Open |
| 7 | 90 | JORDA, Deniasha | SR | Auburndale | NT | |
| 8 | 196 | HOLMES, Berkley | SO | McKeel | 1:14.81 | Lake Region Open |