| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | WELLS, Caroline | Winter Springs | 5:18.69 |
| 2 | PECK, Mary | Father Lopez | 5:30.33 |
| 3 | JOHANSSON, Nina | Winter Park | 5:42.50 |
| 4 | URUCHURTA , Fernanda | Montverde Academy | 5:45.00 |
| 5 | NESMITH, Natalie | Montverde Academy | 5:46.00 |
| 6 | WHITE, Karah | Montverde Academy | 5:47.00 |
| 7 | MEANS, Ally | Oak Hall | 5:50.00 |
| 8 | FLOWERS, Naomi | Winter Springs | 5:50.43 |
| 9 | LANTZ, Sofia | Lake Mary | 5:51.67 |
| 10 | EISNER, Eliana | Oak Hall | 5:53.06 |
| 11 | GREZ, Brenda | Timber Creek | 5:57.58 |
| 12 | WINN, Lyra | Lake Mary | 5:58.63 |
| 13 | THOMAS, Zoey | Winter Park | 5:59.05 |
| 14 | MULLANEY, Grace | Bishop Kenny | 6:01.05 |
| 15 | BADILLO, Jaden | Windermere | 6:01.78 |
| 16 | MULLIGAN, Mackenzie | Tavares | 6:03.68 |
| 17 | ROSARIO, Araceliz | Colonial | 6:06.66 |
| 18 | GREENFIELD, Mia | Windermere | 6:07.14 |
| 19 | MCMAHAN, Emma | Bishop Kenny | 6:09.74 |
| 20 | CANAVAN, Amber | Bishop Kenny | 6:10.48 |
| 21 | PERRY, Brooke | Lake Mary | 6:11.00 |
| 22 | SZYNAKA, Bella | Winter Springs | 6:17.50 |
| 23 | MEDINA-TORRES, Amanda | Hagerty | 6:17.89 |
| 24 | KAY, Jillian | Vanguard (Ocala) | 6:18.54 |
| 25 | LAMMENS, Emma | Vanguard (Ocala) | 6:28.00 |
| 26 | SANDER, Emilly | Lake Howell | 6:29.53 |
| 27 | GOMEZ, Katie | Lake Howell | 6:30.34 |
| 28 | OWJI, Nikki | Oviedo | 6:33.57 |
| 29 | KIRBY, Taylor | Winter Park | 6:35.50 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | WYANT, Ava | Master's Academy (Oviedo) | 6:37.00 |
| 2 | ROBERTS, Logan | Vanguard (Ocala) | 6:38.00 |
| 3 | BIGNEY, Jaiden | Trinity Prep | 6:41.66 |
| 4 | HUPTON, Jasmine | Lyman | 6:42.93 |
| 5 | CANNELLA, Jessica | Boone | 6:44.21 |
| 6 | GALLAGHER, Emily | Lake Highland Prep | 6:46.01 |
| 7 | AKINS, Macy | Lake Highland Prep | 6:50.71 |
| 8 | MARTINEZ, Kayla | Colonial | 6:56.67 |
| 9 | CACERES, Valeria | Timber Creek | 7:02.33 |
| 10 | SMITH, Dylan | Tavares | 7:04.10 |
| 11 | CHANDLER, Lydia | Tavares | 7:06.45 |
| 12 | NAVARRO, Alandra | West Orange | 7:06.77 |
| 13 | OLSEN, Amelie | Dr. Phillips | 7:09.67 |
| 14 | BOKTOR, Stephanie | Master's Academy (Oviedo) | 7:10.67 |
| 15 | JEAN SIMON, Shernika | Cypress Creek | 7:11.96 |
| 16 | OBERSCHLAKE, Zoe | Lyman | 7:15.22 |
| 17 | KEEFE, Leah | Trinity Prep | 7:32.73 |
| 18 | DIAZ, Keilani | Colonial | 7:33.22 |
| 19 | TORRES, Paola | Cypress Creek | 7:35.31 |
| 20 | KORTMAN, Ava | Trinity Prep | 7:53.20 |
| 21 | CHHU, Emely | Lake Howell | NT |
| 22 | CIRINEO, Sophie | Dr. Phillips | NT |
| 23 | DELGADO, Allianie | East River | NT |
| 24 | FIGUEROA, Alyssa | East River | NT |
| 25 | FRUHWIRTH, Danielle | Oviedo | NT |
| 26 | GEORGE, Ashley | Lake Highland Prep | NT |
| 27 | JENKINS, Lindsay | Oak Hall | NT |
| 28 | NEGRON, Ylannis | Boone | NT |
| 29 | SERRANO, Gabriella | Master's Academy (Oviedo) | NT |