| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 850 | KERN, Julianna | SO | Apopka | 19.10 | Metro Conference Freshman - Sophomore Championship 2019 |
| 2 | 773 | PHILLIPS, Jordian | SR | Olympia | 18.37 | Windermere Meet of Champions 2019 |
| 3 | 6 | FRANKLIN, Destini | JR | Mainland | 17.69 | DeLand/Deltona Meet #2 |
| 4 | 549 | PEREA, Tatiana | JR | Lake Brantley | 17.44 | Spruce Creek George Janan Invitational |
| 5 | 98 | COOPER, Jourdon | JR | Mainland | 17.49 | DeLand/Deltona Meet #2 |
| 6 | 771 | MILLER, Aysha | SO | Olympia | 17.99 | Metro Conference Freshman - Sophomore Championship 2019 |
| 7 | 794 | DACOSTA-MOORE, Dominique | JR | Olympia | 18.40 | Windermere Meet of Champions 2019 |
| 8 | 841 | FOWLER, Hailey | FR | Apopka | 19.61 | Bob Mosher Wildcat Open 2019 |
| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 2 | 488 | MILLER, Emma | JR | Lake Brantley | 21.46 | Kowboy Invitational 2019 |
| 3 | 444 | SYPHAX, Tunisia | SR | Ocoee | 20.85 | Spruce Creek George Janan Invitational |
| 4 | 181 | FIUMARA, Justina | SR | Lake Howell | 19.96 | Brian Jaeger Elite Classic 2019 |
| 5 | 105 | KLEIN, Summer | JR | Mainland | 20.10 | Jeff Wentworth Relays 2019 |
| 6 | 170 | SANDER, Emilly | FR | Lake Howell | 20.97 | SAC Freshman-Sophomore Championship 2019 |
| 7 | 169 | WILLIAMS, Arieuna | FR | Lake Howell | 21.47 | SAC Freshman-Sophomore Championship 2019 |
| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 3 | 511 | PENA, Ana | JR | Lake Brantley | NT | |
| 4 | 731 | HARRIS, Cierra | SR | Deltona | 22.06 | DeLand/Deltona Meet #2 |
| 5 | 828 | PENFIELD, Skylar | SO | Apopka | NT |