| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 867 | PIERROT, Jack | JR | Apopka | 45.36 | Bob Mosher Wildcat Open 2019 |
| 2 | 121 | TAYLOR, Marseille | SO | First Academy (Orlando) | 44.85 | Royal Open 2019 |
| 3 | 872 | JOSEPH, Anthony | FR | Apopka | 43.86 | Windermere Meet of Champions 2019 |
| 4 | 376 | DIX, Avion | SR | Colonial | 40.45 | Sanford Seminole Open at the NTC |
| 5 | 108 | COOPER, Lindon | SR | Mainland | 40.90 | East Coast Classic |
| 6 | 638 | MCKELL, Noah | SR | Wekiva | 44.18 | Bob Mosher Wildcat Open 2019 |
| 7 | 482 | BITTINGER, Kaiden | SO | Lake Brantley | 45.00 | |
| 8 | 545 | BUTTERFIELD, Miles | FR | Lake Brantley | 45.42 | Spruce Creek George Janan Invitational |
| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 342 | ESTRADA, Rodrigo | SR | Colonial | 50.32 | |
| 2 | 411 | CONWAY, Quillian | SR | Ocoee | 47.32 | Spruce Creek George Janan Invitational |
| 3 | 217 | COLLINS, Malik | SO | Lake Howell | 46.74 | Bob Mosher Wildcat Open 2019 |
| 4 | 209 | MOSLEY, Jaquvais | SO | Lake Howell | 45.81 | Royal Open 2019 |
| 5 | 636 | LYONS, Caden | JR | Wekiva | 46.52 | Kowboy Invitational 2019 |
| 6 | 436 | HARRISON, Alan | SO | Ocoee | 47.32 | Spruce Creek George Janan Invitational |
| 7 | 446 | NELSON , Tyler | SO | Ocoee | 48.14 | Kowboy Invitational 2019 |
| 8 | 521 | ANTALEK, Ryan | SR | Lake Brantley | 53.18 | Windermere Meet of Champions 2019 |
| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 3 | 854 | LOWMAN, Jaquan | SO | Apopka | NT | |
| 4 | 722 | NICKERSON, Kenny | FR | Deltona | 56.94 | 5-Star Conference Championship |
| 5 | 713 | ROLON, Kenny | SO | Deltona | NT | |
| 6 | 292 | LOVINSKY, Marc | JR | Freedom (Orlando) | NT |