| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 841 | FOWLER, Hailey | FR | Apopka | 55.37 | Bob Mosher Wildcat Open 2019 |
| 2 | 6 | FRANKLIN, Destini | JR | Mainland | 54.25 | East Coast Classic |
| 3 | 740 | RILEY, Cairah | SO | Olympia | 50.29 | Bob Mosher Wildcat Open 2019 |
| 4 | 110 | ELDER, Jordan | JR | Mainland | 47.83 | 5-Star Conference Championship |
| 5 | 771 | MILLER, Aysha | SO | Olympia | 50.22 | Windermere Meet of Champions 2019 |
| 6 | 549 | PEREA, Tatiana | JR | Lake Brantley | 52.26 | Spruce Creek George Janan Invitational |
| 7 | 98 | COOPER, Jourdon | JR | Mainland | 55.22 | East Coast Classic |
| 8 | 886 | CLARKE, Isis | SO | Apopka | 56.85 | Windermere Meet of Champions 2019 |
| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 731 | HARRIS, Cierra | SR | Deltona | NT | |
| 2 | 225 | CORCINO MILLAN, Lirisha | SO | Lake Howell | 1:02.68 | Royal Open 2019 |
| 3 | 170 | SANDER, Emilly | FR | Lake Howell | 59.94 | Lake Howell QUAD 2019 |
| 4 | 488 | MILLER, Emma | JR | Lake Brantley | 58.03 | Kowboy Invitational 2019 |
| 5 | 444 | SYPHAX, Tunisia | SR | Ocoee | 58.73 | Windermere Meet of Champions 2019 |
| 6 | 828 | PENFIELD, Skylar | SO | Apopka | 1:01.86 | Metro Conference Freshman - Sophomore Championship 2019 |
| 7 | 226 | COOPER, Lylnya | SO | Lake Howell | 1:02.82 | Brian Jaeger Elite Classic 2019 |
| 8 | 416 | LEGER, Esthere | SO | Ocoee | NT |