| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 611 | TOWNSEND, Jocelyn | FR | Wekiva | 27.72 | Kowboy Invitational 2019 |
| 2 | 124 | DAVIS, Sharya | JR | First Academy (Orlando) | 26.85 | Kowboy Invitational 2019 |
| 3 | 117 | AFRAM-GYENING, Courtney | JR | First Academy (Orlando) | 26.67 | Kowboy Invitational 2019 |
| 4 | 60 | WRIGHT, Cydney | SO | Mainland | 24.25 | East Coast Classic |
| 5 | 15 | GRAVES, Acura | JR | Mainland | 25.65 | East Coast Classic |
| 6 | 646 | TEAGUE, Eriana | JR | Wekiva | 26.73 | Bob Mosher Wildcat Open 2019 |
| 7 | 835 | POWELL, Jamiria | SO | Apopka | 27.00 | |
| 8 | 840 | JONES, Hannah | FR | Apopka | 28.16 | Metro Conference Freshman - Sophomore Championship 2019 |
| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 657 | BELL, Trinity | SR | Wekiva | 30.13 | Royal Open 2019 |
| 2 | 464 | VANTERPOOL, Alisha | JR | Lake Brantley | 29.83 | Kowboy Invitational 2019 |
| 3 | 294 | HOWELL, Khalia | SO | Freedom (Orlando) | 29.08 | 44th Annual Eagle Invitational |
| 4 | 827 | JENKINS, Aneicia | JR | Apopka | 28.50 | |
| 5 | 795 | ROUNDTREE-FAIL, Ava | JR | Olympia | 28.63 | Bob Mosher Wildcat Open 2019 |
| 6 | 727 | SLATE, Essence | SO | Deltona | 29.25 | 5-Star Conference Championship |
| 7 | 451 | THOMAS, Jada | FR | Ocoee | 30.00 | Bob Mosher Wildcat Open 2019 |
| 8 | 711 | PHELPS, Kayley | FR | Deltona | 31.26 | Spruce Creek George Janan Invitational |
| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 517 | PATTERSON, J'aria | FR | Lake Brantley | NT | |
| 2 | 254 | JOSEPH, Ruth | JR | Freedom (Orlando) | NT | |
| 3 | 206 | BROWN, Ariyana | SO | Lake Howell | 33.58 | Bob Mosher Wildcat Open 2019 |
| 4 | 496 | THURSTON, Kaley | FR | Lake Brantley | 31.90 | SAC Freshman-Sophomore Championship 2019 |
| 5 | 801 | RODGERS, Nubia | FR | Olympia | 33.26 | Royal Open 2019 |
| 6 | 408 | RENELIQUE, Ashlyne | FR | Ocoee | 33.83 | Spruce Creek George Janan Invitational |
| 7 | 461 | HICKS, Aiyanna | FR | Ocoee | NT | |
| 8 | 271 | MONTILLA, Isabella | JR | Freedom (Orlando) | NT |
| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 3 | 725 | MASON, Aaliyah | JR | Deltona | NT | |
| 4 | 227 | IBLE, Shahnia | SR | Lake Howell | NT | |
| 5 | 220 | WILDER, Ashley | SR | Lake Howell | NT |