| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 555 | BING, Chance | SR | Lake Brantley | 53.00 | |
| 2 | 148 | COX, Allen | JR | First Academy (Orlando) | 51.20 | |
| 3 | 387 | ANDINO, Noel | SO | Colonial | 51.00 | Saint Cloud Invitational 2019 |
| 4 | 1 | REED, Malcolm | SR | Mainland | 50.20 | 5-Star Conference Championship |
| 5 | 667 | JONES, Jesaiah | SO | Wekiva | 50.95 | Royal Open 2019 |
| 6 | 385 | LATOUCHE, Chase | JR | Colonial | 51.13 | Saint Cloud Invitational 2019 |
| 7 | 219 | BYRD, Kevin | JR | Lake Howell | 52.70 | |
| 8 | 668 | FLETCHER, Charles | SO | Wekiva | 53.43 | Royal Open 2019 |
| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 232 | PEREZ-CAMACHO, Harris | JR | Freedom (Orlando) | 54.96 | 44th Annual Eagle Invitational |
| 2 | 424 | CLERJUSTE, David | JR | Ocoee | 54.25 | Windermere Meet of Champions 2019 |
| 3 | 454 | WOOD, Tavion | JR | Ocoee | 54.03 | Spruce Creek George Janan Invitational |
| 4 | 822 | MARTIN, Jaden | SR | Apopka | 53.50 | |
| 5 | 443 | SHERWOOD, Eric | SO | Ocoee | 53.84 | Windermere Meet of Champions 2019 |
| 6 | 864 | TREASURE, Jalen | JR | Apopka | 54.20 | |
| 7 | 16 | FORD, Besean | SR | Mainland | 54.63 | 5-Star Conference Championship |
| 8 | 491 | NOEL, Redwimy | SO | Lake Brantley | 55.31 | SAC Freshman-Sophomore Championship 2019 |
| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 655 | CARTER, Malik | JR | Wekiva | 57.23 | Bob Mosher Wildcat Open 2019 |
| 2 | 787 | LANAHAN, Gryphon | JR | Olympia | 56.50 | Windermere Meet of Champions 2019 |
| 3 | 505 | SHERLOCK, Daniel | JR | Lake Brantley | 56.00 | |
| 4 | 143 | MCARDLE, Tyler | SO | First Academy (Orlando) | 55.44 | Boone HS Quad 2019 |
| 5 | 846 | WILLIAMS, Cornelius | SR | Apopka | 55.93 | Bob Mosher Wildcat Open 2019 |
| 6 | 180 | PALERMO RUIZ, Anthony | SO | Lake Howell | 56.17 | Brian Jaeger Elite Classic 2019 |
| 7 | 192 | PASTRANA, Christian | SO | Lake Howell | 56.57 | SAC Freshman-Sophomore Championship 2019 |
| 8 | 745 | ERNEST, Lantz | JR | Olympia | 58.59 | Royal Open 2019 |
| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 49 | GREENE, Marcus | JR | Mainland | NT | |
| 2 | 730 | LANDAWAY, Alex | FR | Deltona | NT | |
| 3 | 705 | HAIR, Zane | SO | Deltona | NT | |
| 4 | 279 | PINEDA GARCIA, Michael | FR | Freedom (Orlando) | 59.95 | Metro Conference Freshman - Sophomore Championship 2019 |
| 5 | 792 | HOLLIS, Kolby | JR | Olympia | 1:04.99 | Royal Open 2019 |
| 6 | 721 | TEJADA, Freddy | FR | Deltona | NT | |
| 7 | 135 | SUMMERS, Kamron | JR | First Academy (Orlando) | NT | |
| 8 | 330 | VELEZ, Christian | SO | Freedom (Orlando) | NT |