| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 224 | WATSON, Makayla | FR | Lake Howell | 1:03.65 | SAC Freshman-Sophomore Championship 2019 |
| 2 | 122 | SIMPSON, Ella | JR | First Academy (Orlando) | 1:02.36 | Boone HS Quad 2019 |
| 3 | 117 | AFRAM-GYENING, Courtney | JR | First Academy (Orlando) | 1:00.19 | Bob Mosher Wildcat Open 2019 |
| 4 | 646 | TEAGUE, Eriana | JR | Wekiva | 58.00 | |
| 5 | 97 | HAWRYLUK, Grace | JR | Mainland | 1:00.00 | |
| 6 | 412 | CROKER, Zy'Kerriah | SO | Ocoee | 1:01.63 | Spruce Creek George Janan Invitational |
| 7 | 366 | ARROYO, Leyshka | FR | Colonial | 1:03.07 | Bob Mosher Wildcat Open 2019 |
| 8 | 70 | HOWARD, Emanni | FR | Mainland | 1:04.00 |
| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 508 | PENNANT, Cadeeja | SR | Lake Brantley | 1:07.19 | Windermere Meet of Champions 2019 |
| 2 | 422 | SHERWOOD, Eriona | SO | Ocoee | 1:06.38 | Metro Conference Freshman - Sophomore Championship 2019 |
| 3 | 254 | JOSEPH, Ruth | JR | Freedom (Orlando) | 1:05.41 | 44th Annual Eagle Invitational |
| 4 | 835 | POWELL, Jamiria | SO | Apopka | 1:04.10 | |
| 5 | 812 | GRAY, Aalliya | FR | Olympia | 1:05.00 | |
| 6 | 111 | BERRY, Lawren | SR | Mainland | 1:06.14 | East Coast Classic |
| 7 | 878 | BRADLEY, Jordyn | SO | Apopka | 1:07.01 | Windermere Meet of Champions 2019 |
| 8 | 437 | DANIELS, Achante | JR | Ocoee | 1:08.88 | Spruce Creek George Janan Invitational |
| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 719 | PULIDO, Karen | FR | Deltona | 1:17.98 | 5-Star Conference Championship |
| 2 | 715 | MCCAMBRIDGE, Savannah | FR | Deltona | 1:14.34 | Spruce Creek George Janan Invitational |
| 3 | 779 | ELIASSAINT, Veronitte | JR | Olympia | 1:12.23 | Royal Open 2019 |
| 4 | 711 | PHELPS, Kayley | FR | Deltona | 1:11.06 | Spruce Creek George Janan Invitational |
| 5 | 534 | JENKINS, Halie | JR | Lake Brantley | 1:11.65 | George Jenkins Invitational |
| 6 | 890 | SMOTHERS, Zakaria | SO | Apopka | 1:12.63 | Metro Conference Freshman - Sophomore Championship 2019 |
| 7 | 511 | PENA, Ana | JR | Lake Brantley | 1:15.39 | Kowboy Invitational 2019 |
| 8 | 699 | PIERRE, Sara | SO | Wekiva | NT |
| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 3 | 163 | SANTIAGO-RODRIGUEZ, Yarelis | FR | Lake Howell | NT | |
| 4 | 169 | WILLIAMS, Arieuna | FR | Lake Howell | NT | |
| 5 | 233 | SERNA, Isabella | JR | Freedom (Orlando) | NT | |
| 6 | 296 | REID, Bryanna | JR | Freedom (Orlando) | NT |