| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | MOLENDA, Devin | Mount Dora | 44.55 | Mount Dora Quad Meet 2 |
| 2 | MOBLEY, Jaylin | Seminole (Sanford) | 44.13 | Highlander Invitational 2019 |
| 3 | WATTS, Robert | Trinity Prep | 42.53 | Brian Jaeger Elite Classic 2019 |
| 4 | BERTRAN, Sage | Winter Park | 38.34 | Brian Jaeger Elite Classic 2019 |
| 5 | LOPEZ, Vincent | Master's Academy (Oviedo) | 41.47 | Royal Open 2019 |
| 6 | MANZOOR, Ashhad | Winter Park | 43.46 | Brian Jaeger Elite Classic 2019 |
| 7 | LEAK, Yusef | Master's Academy (Oviedo) | 44.32 | Royal Open 2019 |
| 8 | DENNIS, Keyon | Seminole (Sanford) | 45.40 | Spruce Creek George Janan Invitational |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | MCDANIEL, Matthew | Master's Academy (Oviedo) | 50.49 | 44th Annual Eagle Invitational |
| 2 | SELLERS, Caleb | Mount Dora | 48.57 | Brian Jaeger Elite Classic 2019 |
| 3 | SIMMONS, William | Hagerty | 46.89 | Highlander Invitational 2019 |
| 4 | DRENNAN, Ephraim | Tavares | 45.98 | Brian Jaeger Elite Classic 2019 |
| 5 | THOMAS, Robert | Seminole (Sanford) | 46.71 | SAC Freshman-Sophomore Championship 2019 |
| 6 | DENNEY, Sean | Bishop Moore | 47.75 | Astronaut developmental meet #2 |
| 7 | GUZMAN, Kioris | Mount Dora | 50.33 | Mount Dora Quad Meet 2 |
| 8 | MURPHY, Donovan | Bishop Moore | 50.59 | Larry Kelly Invitational 2019 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | SKAGE, John | Winter Park | NT | |
| 2 | MCMURRAY, Calvin | Oviedo | 1:01.09 | Lake Howell QUAD 2019 |
| 3 | MEJIA, David | Hagerty | 53.31 | SAC Freshman-Sophomore Championship 2019 |
| 4 | OLIVO, Alejandro | Bishop Moore | 51.32 | Highlander Invitational 2019 |
| 5 | THOMPSON, Jayden | Oviedo | 51.90 | Saint Cloud Invitational 2019 |
| 6 | HENSON, Ron | Hagerty | 54.94 | Highlander Invitational 2019 |
| 7 | RENK, Kyle | Oviedo | NT |