| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | MUKOIE-CARCAMO, Diana | Master's Academy (Oviedo) | 55.88 | Sanford Seminole Open at the NTC |
| 2 | MOURY, Hannah | Circle Christian | 55.11 | Larry Kelly Invitational 2019 |
| 3 | HARRIS, Essence | Jones | 53.08 | Bob Mosher Wildcat Open 2019 |
| 4 | MCCALLA-CLARK, Daimone | Seminole (Sanford) | 16.50 | |
| 5 | JACKSON, Joya | Jones | 49.90 | Bob Mosher Wildcat Open 2019 |
| 6 | ALEXANDRE, Stacy | Jones | 55.01 | Carl Friedland Memorial 2019 |
| 7 | DAVIS, Sarah | Master's Academy (Oviedo) | 55.15 | Royal Open 2019 |
| 8 | GASTON, Nicole | Lake Mary Prep | 56.13 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | MATTADEEN, Isabella | Seminole (Sanford) | 58.64 | Trinity Prep Invitational 2019 |
| 2 | GEHRER, Rebecca | Hagerty | 58.40 | Lake Howell QUAD 2019 |
| 3 | WEST, Alexa | Bishop Moore | 57.31 | Highlander Invitational 2019 |
| 4 | OMALLEY, Meagan | Bishop Moore | 56.30 | Highlander Invitational 2019 |
| 5 | FILDES, Hali | Hagerty | 56.97 | SAC Freshman-Sophomore Championship 2019 |
| 6 | FYOCK, Ashley | Master's Academy (Oviedo) | 57.32 | Royal Open 2019 |
| 7 | AVERY, Caroline | Trinity Prep | 58.64 | Bolles Bulldog Classic |
| 8 | MOORE, Payton | Winter Park | 1:00.12 | Trinity Prep Invitational 2019 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | GALLOWAY, Adrice | Oviedo | 1:02.65 | Carl Friedland Memorial 2019 |
| 3 | YARED, Hannah | Trinity Prep | 1:01.41 | Lake Howell QUAD 2019 |
| 4 | WHITE, Mallory | Winter Park | 1:00.32 | Saint Cloud Invitational 2019 |
| 5 | BRASKO, Brianna | Bishop Moore | 1:01.13 | Highlander Invitational 2019 |
| 6 | RAMPERSAUD, Amber | Trinity Prep | 1:01.65 | Trinity Prep Invitational 2019 |
| 7 | STIGER, Darya Grace | Hagerty | 1:03.32 | SAC Freshman-Sophomore Championship 2019 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | LEE, Destani | Seminole (Sanford) | NT | |
| 4 | KING, Angel | Mount Dora | NT | |
| 5 | DURIAS, Sydney | Mount Dora | NT |