| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | DORCELY, Sagie-May | Bishop Moore | 28.97 | Larry Kelly Invitational 2019 |
| 2 | JACKSON, Kennedy | Bishop Moore | 28.01 | Highlander Invitational 2019 |
| 3 | HENDERSON, Savannah | Timber Creek | 27.80 | Highlander Invitational 2019 |
| 4 | PATRICE, Shenya | Timber Creek | 26.28 | Brian Jaeger Elite Classic 2019 |
| 5 | OSTOPOVICH, Mia | Astronaut | 26.78 | |
| 6 | GODBOLT, Sierra | Lake Highland Prep | 27.80 | |
| 7 | NEWSOME, Grace | Bishop Moore | 28.84 | Astronaut developmental meet #2 |
| 8 | MADDOX, Taylor | Bishop Moore | 29.00 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | MORENO, Ivanna | Winter Springs | 30.36 | Carl Friedland Memorial 2019 |
| 2 | HENDRIX, Lily | Geneva School | 30.00 | |
| 3 | COVARRUBIAS, Briana | Tavares | 29.90 | Larry Kelly Invitational 2019 |
| 4 | WILLIAMS, Kayla | Tavares | 29.37 | Brian Jaeger Elite Classic 2019 |
| 5 | PEREZ, Nadia | Timber Creek | 29.79 | Brian Jaeger Elite Classic 2019 |
| 6 | TREJO, Briana | Tavares | 29.91 | |
| 7 | ACEVEDO, Lilian | Victory Christian (LL) | 30.20 | Chargers Invitational |
| 8 | FOREMAN, Anna | Geneva School | 30.77 | Larry Kelly Invitational 2019 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | RIBBLE, Cansas | Tavares | 31.84 | Brian Jaeger Elite Classic 2019 |
| 2 | WHITTENTON, Kaylie | Victory Christian (LL) | 31.68 | Trinity Prep Invitational 2019 |
| 3 | GREENE, Charnee | Lake Howell | 31.36 | SAC Freshman-Sophomore Championship 2019 |
| 4 | WHEELER, Lana | Lake Highland Prep | 30.96 | Brian Jaeger Elite Classic 2019 |
| 5 | COSTANZO, Paige | Lake Howell | 31.21 | Lake Howell QUAD 2019 |
| 6 | LASALDE, Fabiola | Lake Howell | 31.51 | Lake Howell QUAD 2019 |
| 7 | BINES, Carly | Lake Highland Prep | 31.73 | Highlander Invitational 2019 |
| 8 | PARSONS, Claire | Geneva School | 35.12 | Larry Kelly Invitational 2019 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | GARCIA, Yamilet | Victory Christian (LL) | NT | |
| 4 | HUAMAN, Emma | Winter Springs | 35.28 | SAC Freshman-Sophomore Championship 2019 |
| 5 | LOWRY, Maria | Winter Springs | NT |