| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | Tampa Catholic | A | 44.38 | Cypress Creek Invitational 2019 |
| 3 | Lakeland | A | DNS | Polk County Championship 2019 |
| 4 | Dunbar | A | DNS | |
| 5 | Tampa Bay Tech | A | 42.09 | 57th Annual Ed Wells Jr. - Kiwanis Invitational |
| 6 | Kathleen | A | DQ | Lakeland City Meet 2019 |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | Sunlake | A | 45.65 | Cypress Creek Invitational 2019 |
| 3 | Alonso | A | 45.33 | Wharton Wildcat Invite |
| 4 | George Steinbrenner | A | 44.97 | Steinbrenner Quad-Meet 3-13 |
| 5 | Seffner Christian | A | DNS | Mustang Invitational |
| 6 | Land O'Lakes | A | 45.44 | Cypress Creek Invitational 2019 |