| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Mitchell | A | 4:37.32 | 57th Annual Ed Wells Jr.-Kiwanis Invitational |
| 2 | Wiregrass Ranch | A | 4:19.05 | Kowboy Invitational 2019 |
| 3 | Lake Wales | A | 4:17.21 | Polk County Championship 2019 |
| 4 | Alonso | A | 4:07.34 | 49th Nash Higgins Relays |
| 5 | George Jenkins | A | 4:12.10 | Berkeley Track and Field Invitational |
| 6 | Kathleen | A | DNS | Kowboy Invitational 2019 |