| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 4 | ARCHIBALD-BOWERS, Giselle | Timber Creek | NT | 51.7 |
| 5 | DAVID, Jennay | Lake Nona | 59.75 | Larry Kelly Invitational 2019 |
| 6 | DORCELY, Fisnerline | Oak Ridge | 1:01.82 | Windermere Meet of Champions 2019 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | SMITH, Beonni | East River | 58.52 | Bob Mosher Wildcat Open 2019 |
| 3 | SMITH, Jalaea | Boone | 58.12 | Metro Conference Freshman - Sophomore Championship 2019 |
| 4 | DOGHOR, Lilian | University (Orlando) | 56.51 | Bob Mosher Wildcat Open 2019 |
| 5 | SMITH, Kaela | University (Orlando) | 54.70 | Metro Conference Freshman - Sophomore Championship 2019 |
| 6 | PICHARDO, Arianna | East River | 55.83 | Boone Invitational 2019 |
| 7 | DESHOTEL, Kaitlin | Colonial | 57.45 | Metro Conference Freshman - Sophomore Championship 2019 |
| 8 | DRYDEN, Haiven | Boone | 58.42 | Metro Conference Freshman - Sophomore Championship 2019 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | MOORE, Payton | Winter Park | 54.13 | Boone Invitational 2019 |
| 2 | RODRIGUEZ, Yessmary | Colonial | 53.29 | Brian Jaeger Elite Classic 2019 |
| 3 | COLON, Stephanie | University (Orlando) | 52.51 | Spruce Creek George Janan Invitational |
| 4 | MARSTON, Marlia | Lake Nona | 51.61 | Larry Kelly Invitational 2019 |
| 5 | DEVLIN, Alexis | Winter Park | 47.25 | Boone Invitational 2019 |
| 6 | VASQUEZ, Aaliyah | Colonial | 49.60 | Brian Jaeger Elite Classic 2019 |
| 7 | BRYANT-BERRY, Zaniyah | Winter Park | 51.81 | Bob Mosher Wildcat Open 2019 |
| 8 | NIEVES, Alexys | Oak Ridge | 53.00 | Sanford Seminole Open at the NTC |
| 9 | FORESTER, Kisha | Lake Nona | 54.08 | Boone Invitational 2019 |